MAP 激酶和 Wnt 途径汇聚在一起,对 Caenorhabditis elegans 中的一种 HMG 域抑制剂进行下调
M D Meneghini1, T Ishitani, J C Carter
1Institute of Molecular Biology, University of Oregon, Eugene 97403, USA.
Nature
|July 3, 1999
概括
Wnt信号传递和与MAPK相关的通路通过降低Caenorhabditis elegans发育中的POP-1抑制剂来调节细胞命运决策. 这种保存机制涉及MOM-4和LIT-1,与脊椎动物TAK1和NLK同类.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 通过控制具有高流动性组 (HMG) 域的转录因子,Wnt信号调节动物的发育.
- 在C. elegans中,HMG-域抑制剂POP-1使前置和后置细胞命运产生差异.
- 通常,Wnt信号会降低特定后端子细胞 (如E) 中的POP-1活性.
研究的目的:
- 研究参与降低POP-1活动的新基因.
- 为了阐明分子途径与Wnt信号合作在细胞命运决定.
- 探索脊椎动物中这种通路的保护.
主要方法:
- 对C. elegans突变的遗传分析.
- 鉴定参与POP-1调节的基因.
- 生物化学测试以表征蛋白质相互作用和激酶活动.
- 与脊椎动物同类动物进行比较分析.
主要成果:
- mom-4和lit-1基因对于在多个后子细胞中降低POP-1的调节至关重要.
- mom-4和lit-1编码的是一个基因激活蛋白激酶 (MAPK) 途径的组成部分,与脊椎动物TAK1和NLK相同.
- MOM-4和TAK1与相关蛋白相互作用,增强它们的激酶活性.
结论:
- 涉及MOM-4和LIT-1的MAPK相关途径与Wnt信号合作,以减少POP-1活动.
- 这条通路在C. elegans发育过程中在细胞命运决策中起着至关重要的作用.
- 这些发现表明脊椎动物中存在保存机制,其中TAK1和NLK可以调节类似的HMG域蛋白.
相关概念视频
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...