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HMG-1作为老鼠内毒素致死性的晚期调解者
1Department of Emergency Medicine and Department of Surgery, North Shore University Hospital-New York University School of Medicine, Manhasset, NY 11030, USA. hwang@picower.edu
Science (New York, N.Y.)
|July 10, 1999
概括
高流动性组-1 (HMG-1) 是内毒素致死性的晚期调解者. 阻断HMG-1保护小鼠免受内毒性,这表明它是.
科学领域:
- 免疫学和分子医学
- 细菌病原和宿主反应
背景情况:
- 格拉姆阴性细菌的内毒素会触发巨细胞释放瘤缩因子 (TNF) 和互白素-1 (IL-1).
- 这些早期媒介可以导致组织损伤和致命的休克 (内毒性病).
- 针对TNF和IL-1的治疗策略在临床上取得了有限的成功,这表明其他病原性因素的参与.
研究的目的:
- 识别和描述可能导致内毒素诱导致死性的晚期调解者.
- 调查高流动性组-1 (HMG-1) 蛋白在内毒性病原发生中的作用.
主要方法:
- 用内毒素,TNF或IL-1刺激巨细胞培养,以评估HMG-1释放动力学.
- 在32小时内暴露于内毒素后,测量了小鼠的血清HMG-1水平.
- 抗HMG-1抗体和HMG-1的有效性在内毒性病的小鼠模型中进行了评估.
主要成果:
- 巨细胞释放的HMG-1蛋白发生在内毒素,TNF或IL-1刺激后8小时以上.
- 在内毒素暴露后8至32小时的小鼠中观察到血清HMG-1水平升高.
- 延迟使用抗HMG-1抗体显著降低了小鼠内毒素诱导的致死率.
- 在小鼠模型中,HMG-1的直接给药被证明是致命的.
结论:
- 在内毒素诱导的致死性中,HMG-1充当了迟起作用的调解者.
- 在治疗上准HMG-1可能为治疗内毒性病提供一种新的策略.
- 受感染而死亡的败血症患者中HMG-1水平升高支持其在人类败血症中的作用.
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Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...