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氧化有助于行为,细胞和发育对低氧的反应在Drosophila中

J A Wingrove¹, P H O'Farrell

¹Department of Biochemistry and Biophysics, University of California, San Francisco, 94143-0448, USA.

|July 21, 1999

概括

虫幼虫使用氧化 (NO) /循环GMP (cGMP) 途径来应对低氧气. 这条通路调节了缺氧期间的行为,细胞循环和生存.

科学领域:

细胞和分子生物学是细胞和分子生物学.
身体生理学身体生理学
动物模型动物模型

背景情况:

氧化 (NO) /循环GMP (cGMP) 途径对于低氧条件下的哺乳动物的血管扩张至关重要.
了解物种之间保存的信号通路可以揭示基本的生物机制.

研究的目的:

研究NO/cGMP途径在Drosophila对低氧反应中的作用.
为了确定这种保存的途径是否在无脊椎动物中用于缺氧反应.

主要方法:

多虫幼虫的低毒性暴露.
抑制NO合成酶和对cGMP依赖蛋白激酶的基因操纵.
对NO合成酶的异胎表达.
评估行为和细胞反应,气管发育和生存率.

主要成果:

低氧诱导了幼虫的探索行为和细胞循环停止.
抑制NO合成酶或改变cGMP依赖的蛋白激酶降低了这些反应.
宫外NO合成酶表达模仿了缺氧反应.
NO/cGMP通路在长期缺氧期间影响了气管发育和生存.

结论:

草使用NO/cGMP通路的组件来适应低毒环境.
NO和蛋白质激酶G是Drosophila对缺氧反应的关键媒介.
这条通路在调节行为,细胞循环和缺氧下生存方面发挥着重要作用.

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