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相关概念视频

Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...

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Updated: Jul 12, 2026

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
11:25

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

通过 LEAFYY 对APETALA1的转录激活.

D Wagner1, R W Sablowski, E M Meyerowitz

  • 1California Institute of Technology, Division of Biology 156-29, Pasadena, CA 91125, USA.

Science (New York, N.Y.)
|July 27, 1999
PubMed
概括
此摘要是机器生成的。

植物中的花转换是由LEFY (LFY) 调节的,它直接诱导APETALA1 (AP1) 基因表达. 这一关键步骤对于花的发育至关重要,发生在早期,并且独立于蛋白质合成.

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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
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Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

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Last Updated: Jul 12, 2026

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
11:25

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

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科学领域:

  • 植物发育生物学植物发育生物学
  • 分子遗传学 分子遗传学
  • 花的器官生成 植物的器官生成

背景情况:

  • 植物产生新的结构从发芽的角干细胞,这是干细胞种群.
  • 从植物生长到开花的过渡是一个关键的发育转换.
  • 像LEFY (LFY) 和APETALA1 (AP1) 这样的关键调节者控制着花的发育.

研究的目的:

  • 调查在花过渡期间LEFY (LFY) 和APETALA1 (AP1) 之间的调节关系.
  • 通过LFY.确定AP1基因诱导的机制和时间.

主要方法:

  • 使用类固醇诱导系统来控制LFY表达.
  • 在对LFY激活的反应中分析AP1的基因表达模式.
  • 研究蛋白质合成在AP1诱导中的作用.

主要成果:

  • APETALA1 (AP1) 的早期表达是直接通过 LEAFY (LFY) 转录诱导的.
  • 这种由LFY诱导AP1的发生独立于新的蛋白质合成.
  • 通过LFY诱导AP1是特定于获得花命运的组织和发育时间.
  • 后来的AP1表达似乎受到LFY的间接影响.

结论:

  • 叶子 (LFY) 在花过渡期间作为APETALA1 (AP1) 的直接转录激活剂.
  • 由LFY早期的,蛋白质合成独立的AP1诱导是建立花身份的关键事件.
  • 在AP1调节中LFY的作用在花发育过程中从直接转向间接.