在体外循环期间单细胞激活中C3裂变的作用
C S Rinder1, H M Rinder, K Johnson
1Department of Laboratory Medicine and Anesthesiology, Yale University School of Medicine, New Haven, CT 06520-8035, USA. christine.rinder@yale.edu
Circulation
|August 3, 1999
概括
阻断早期补体激活,特别是C3a,可以防止单细胞在模拟体外循环 (SECC) 期间激活. 这与之前的发现形成鲜明对比,只有终端补充抑制阻断了血小板和中性粒细胞的激活.
科学领域:
- 免疫学 免疫学 免疫学
- 通过心肺绕道术 (cardiopulmonary bypass) 进行心肺绕道.
- 补充系统 补充系统
背景情况:
- 以前的研究表明,终端补充抑制 (C5a,C5b-9) 阻断了血小板和中性粒细胞激活,但在SECC期间没有单细胞激活.
- 这项研究调查了早期的补充抑制,通过阻断C3a,是否可以防止单细胞激活.
研究的目的:
- 为了确定在SECC期间阻断C3a形成是否可以防止单细胞激活.
- 为了进一步了解在模拟心肺绕道术期间补体激活和血细胞激活之间的关系.
主要方法:
- 模拟体外循环 (SECC) 模型使用人类血液和膜氧化器.
- 服用CAB-2,一种抑制C3/C5转化酶的化学蛋白质,以阻止C3a,C5a和C5b-9的形成.
- 对补充成分 (C3a,C5b-9),单细胞激活 (CD11b),中性粒细胞激活 (CD11b,弹性酶) 和血小板激活 (P-选择素,结合物) 的测试.
主要成果:
- 在两种测试剂量下,CAB-2显著抑制了C3a和C5b-9的形成.
- 高剂量的CAB-2显著阻断单细胞和中性粒细胞CD11b上调和中性粒细胞弹性酶释放.
- CAB-2还抑制了依赖于血小板激活的单细胞-血小板结合物的形成.
结论:
- 早期的补充阻断 (C3a抑制) 在SECC期间阻止单细胞CD11b上调.
- 早期补充成分对于SECC中单细胞激活至关重要.
- 这项研究阐明了补充系统在模拟心肺绕行术期间血液细胞激活中的作用.
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