相关实验视频
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Live Cell Imaging during Mechanical Stretch
Published on: August 19, 2015
细胞运动的调节是由拉伸激活通道调节的
1Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090, USA. jlee@uconnvm.uconn.edu
Nature
|August 4, 1999
概括
细胞过渡体在运动过程中信号机械应力. 这种机制涉及拉伸激活通道,有助于细胞脱离表面并调节运动.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 机械生物学 机械生物学
背景情况:
- 细胞内 ([Ca2+]i) 对于细胞运动,调节收缩力,行为动力学和细胞基质粘附至关重要.
- 在各种信号传导途径中充当第二信使,但其在整体细胞运动调节中的确切作用尚不清楚.
研究的目的:
- 阐明细胞内调节整体细胞运动的机制.
- 为了研究机械力量在触发细胞移动过程中的过渡的作用.
主要方法:
- 研究了鱼类上皮质角质细胞的运动.
- 在不同的条件下 (例如,基质粘附,机械拉伸) 监测细胞移动期间的细胞内过渡物 ([Ca2+]i).
- 研究了拉伸激活通道和细胞外流入的作用.
主要成果:
- 细胞内 ([Ca2+]i) 的过渡性增加在暂时粘在基质或机械拉伸的角质细胞中更频繁地发生.
- 过渡性被拉伸激活通道的激活触发,导致细胞外流入.
- [Ca2+]i的增加与后细胞边缘脱落有关.
结论:
- 他们发现了一种新的机制,细胞通过过渡体检测并将机械力转化为生物化学信号.
- 这种信号通路通过促进细胞在机械应力下脱离基质来调节细胞的运动.
- 伸展激活通道在将机械刺激与调节细胞运动的细胞反应联系起关键作用.
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