Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Caspases01:24

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Radiographic Characteristics of the Femoral Nutrient Artery Canals in Total Hip Arthroplasty using Cementless Femoral Stem.

Malaysian orthopaedic journal·2023
Same author

Pericardial diverticulum arising from the right lateral superior aortic recess: a mimicker of cystic anterior mediastinal mass.

Clinical radiology·2023
Same author

Focal Myositis Localised in Gastrocnemius Muscle: is it Different from Isolated Gastrocnemius Myositis? A Case Report.

Malaysian orthopaedic journal·2021
Same author

Reconstitution of Human Necrosome Interactions in <i>Saccharomyces cerevisiae</i>.

Biomolecules·2021
Same author

Effects of Inflammatory Disease on Clinical Progression and Treatment of Ischiogluteal Bursitis: A Retrospective Observational Study.

Malaysian orthopaedic journal·2021
Same author

ICRP Publication 144: Dose Coefficients for External Exposures to Environmental Sources.

Annals of the ICRP·2020

相关实验视频

Updated: Jul 6, 2026

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
13:21

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

德罗斯菲拉酶抑制剂DIAP1对于细胞存活至关重要,并且受到HID的负面调节.

S L Wang1, C J Hawkins, S J Yoo

  • 1Division of Biology MC 156-29, California Institute of Technology, Pasadena 91125, USA.

Cell
|September 11, 1999
PubMed
概括
此摘要是机器生成的。

多虫收割者 (RPR),头部卷积缺陷 (HID) 和GRIM通过破坏与细胞死亡抑制剂DIAP1的相互作用来促进细胞亡. DIAP1对于阻断酶活性和防止胚胎细胞死亡至关重要.

更多相关视频

Detecting Anastasis In Vivo by CaspaseTracker Biosensor
20:16

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Published on: February 1, 2018

In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
08:16

In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening

Published on: October 6, 2022

相关实验视频

Last Updated: Jul 6, 2026

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
13:21

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

Detecting Anastasis In Vivo by CaspaseTracker Biosensor
20:16

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Published on: February 1, 2018

In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
08:16

In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening

Published on: October 6, 2022

科学领域:

  • 细胞生物学 细胞生物学
  • 发育生物学是发展生物学.
  • 遗传学 是一个遗传学.

背景情况:

  • 卡斯巴酶依赖的亡对于发育至关重要.
  • 亡蛋白抑制剂 (IAP),就像DIAP1一样,可以调节亡.
  • 收割者 (RPR),头部卷积缺陷 (HID) 和GRIM是Drosophila中的亲亡蛋白.

研究的目的:

  • 调查RPR,HID和GRIM诱导亡的机制.
  • 阐明这些蛋白质与细胞死亡抑制剂DIAP1.1之间的相互作用.
  • 了解DIAP1在调节酶活性和细胞存活中的作用.

主要方法:

  • 生物化学试验用于研究蛋白质相互作用.
  • 在Drosophila中进行基因操纵以评估蛋白质功能.
  • 对酶活性和胚胎死亡率的分析.

主要成果:

  • 头部卷积缺陷 (HID) 已被证明可以抑制DIAP1的caspase抑制功能.
  • 有证据表明,Reaper (RPR) 和GRIM的行为与HID.类似.
  • 消除DIAP1功能导致了广泛的胚胎细胞死亡和增加的酶活性.
  • 即使在没有RPR,HID和GRIM的情况下,DIAP1仍然对细胞生存至关重要.

结论:

  • 通过干扰IAP-caspase相互作用,RPR,HID和GRIM促进了亡.
  • DIAP1对于抑制诱导亡的酶活性和确保细胞存活至关重要.
  • RPR/HID/GRIM和DIAP1之间的相互作用是Drosophila发育中的关键调节机制.