Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Position-effect Variegation02:32

Position-effect Variegation

5.6K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
5.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.1K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.1K
Polytene Chromosomes02:04

Polytene Chromosomes

9.3K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
9.3K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.0K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.0K
Dosage Compensation02:50

Dosage Compensation

6.4K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with  distinct numbers of X chromosomes will...
6.4K
Exon Recombination02:32

Exon Recombination

3.1K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Introduction to the special issue on Environmental Neurology.

Journal of the neurological sciences·2026
Same author

(5,3) SWCNT-doping phosphorus-magnesium: Difference in adsorption of cardiovascular drugs.

Journal of molecular graphics & modelling·2025
Same author

Study of clopidogrel and clonidine interactions for cardiovascular formulations: progress from DFT modeling.

Nanoscale advances·2025
Same author

AMINO ACIDS METABOLOMIC SIGNATURE OF BLOOD PRESSURE VARIABILITY In Type 2 Diabetes.

Acta endocrinologica (Bucharest, Romania : 2005)·2023
Same author

METABOLOMIC ANALYSIS OF NORMAL WEIGHT, HEALTHY AND UNHEALTHY OBESITY: AMINO ACID CHANGE ACROSS THE SPECTRUM OF METABOLIC WELLBEING IN WOMEN.

Acta endocrinologica (Bucharest, Romania : 2005)·2022
Same author

Relevance of silica surface morphology in Ampyra adsorption. Insights from quantum chemical calculations.

RSC advances·2022

相关实验视频

Updated: May 5, 2026

Chromatin Immunoprecipitation ChIP using Drosophila tissue
13:47

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Published on: March 23, 2012

24.6K

从roXRNA基因传播Drosophila剂量补偿复合物的表观遗传扩散到侧面的染色质.

R L Kelley1, V H Meller, P R Gordadze

  • 1Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.

Cell
|September 11, 1999
PubMed
概括

在雄性Drosophila X染色体上的MSL复合体使用roXRNA作为进入点. 这使得剂量补偿复合体能够结合和超转录X染色体.

科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 染色体的调节方式

背景情况:

  • 在Drosophila中,雄性X染色体被超转录以使基因剂量与雌性相匹配.
  • 这个过程是由MSL (男性特异性致死性) 剂量补偿复合体介导的.
  • 非编码RNAs称为roX (RNA在X上) 也在男性X染色体上发现.

研究的目的:

  • 为了研究roXRNAs在MSL复杂结合中的功能.
  • 了解MSL复合体如何识别和结合X染色体.
  • 为了阐明Drosophila中剂量补偿的机制.

主要方法:

  • 对MSL复合体结合在msl3,mle和mof的突变体中的分析.
  • 确定roX1和roX2基因作为MSL复合体的结合部位.
  • 对roX1基因位置进行实验性操纵,以评估MSL复合物的扩散.

主要成果:

  • 在msl3,mle或mof的突变导致MSL复合体在约35个位点的部分结合.
  • 其中两个位点是roX1和roX2基因,这表明它们充当了入口点.
  • roX1基因可以核化MSL复合体,扩散到侧面染色质,甚至在自体和cis或trans上.

更多相关视频

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

22.0K
RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

57

相关实验视频

Last Updated: May 5, 2026

Chromatin Immunoprecipitation ChIP using Drosophila tissue
13:47

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Published on: March 23, 2012

24.6K
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

22.0K
RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

57

结论:

  • roX RNAs对于MSL复合体与X染色体的初始识别和结合至关重要.
  • roX1基因作为核化部位,促进MSL复合体沿X染色体传播.
  • 提出了一个模型,说明剂量补偿复合物如何识别和结合X染色体,其中包括roXRNA作为关键入口点.