通过产生超氧化物的氧化酶Mox1的细胞转化
Y A Suh1, R S Arnold, B Lassegue
1Department of Biochemistry, Emory University Medical School, Atlanta, Georgia 30322, USA.
Nature
|September 15, 1999
概括
反应性氧物种 (ROS) 与癌症和细胞生长有关. 一个新发现的基因mox1在非食细胞中产生ROS,驱动细胞增殖和瘤形成.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 在瘤学瘤学.
背景情况:
- 反应性氧物种 (ROS) 在菌原体信号传递和癌症发展中发挥作用.
- 在许多癌细胞中观察到ROS产量的增加,与增强的增殖相关.
研究的目的:
- 鉴定和表征一种新型基因,该基因参与非食细胞中ROS的产生.
- 研究该基因在细胞生长,增殖和瘤形成中的作用.
主要方法:
- 克隆mox1基因,这是gp91phox.的同类基因.
- 在各种组织中分析mox1mRNA表达.
- 研究mox1操纵 (反感,过度表达) 对ROS生成和细胞生长 in vitro的影响.
- 在体内评估瘤形成,使用无血性小鼠.
主要成果:
- mox1编码一种超氧化物生成的NADPH氧化酶同类物,在结肠,前列腺,子宫和血管光滑肌中表达.
- 血小板衍生生长因子在光滑肌细胞中诱导mox1mRNA.
- 反感性mox1降低了超氧化物生成和生长;mox1过度表达增加了超氧化物生成和细胞生长.
- 在小鼠中,mox1表达细胞表现出转变的表型,独立于 anchorage的生长,并形成瘤.
结论:
- 莫克斯1基因将ROS的产生与非食细胞细胞的生长控制联系起来.
- mox1是细胞增殖的关键调节剂,具有致癌潜力.
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