在3.3A分辨率下,Thermus aquaticus核心RNA聚合酶的晶体结构
G Zhang1, E A Campbell, L Minakhin
1The Rockefeller University, New York, New York 10021, USA.
Cell
|September 28, 1999
概括
热水核RNA聚合酶的晶体结构显示了爪形状,有一个中央通道. 这揭示了一个关键的离子和功能部位,对RNA合成和酶过程性至关重要.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 分子生物学分子生物学
背景情况:
- RNA聚合酶对于所有生物体的基因转录至关重要.
- 了解核心RNA聚合酶的结构是解读其催化机制的关键.
- 水体热 (Thermus aquaticus) 为研究细菌和真核细胞RNA聚合酶提供了一个模型系统.
研究的目的:
- 为了确定Thermus aquaticus核心RNA聚合酶的X射线晶体结构.
- 为了确定催化活性位点的位置和保存的功能动机.
- 阐明涉及转录过程性和基质递送的结构特征.
主要方法:
- 使用X射线晶体学来获得高分辨率的结构.
- 通过突变和交叉链接研究发现的保存型号和功能部位的分析.
- 结构特征与已知的功能角色在转录中的比较.
主要成果:
- 结构显示出"爪"形状,内部通道宽27英里.
- 一个催化必需的Mg2+离子位于通道的后壁上,由保存的图案化.
- 维持转录泡,DNA/RNA结合和基质递送的关键功能部位位于通道内.
结论:
- 确定的结构提供了核心RNA聚合酶功能的详细分子模型.
- 保存的Mg2+结合基因对各种生命形式的催化活性至关重要.
- 结构洞察力解释了RNA聚合酶如何保持过程性并有效合成RNA.
相关概念视频
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
00:43Bacterial RNA Polymerase
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In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
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00:58Eukaryotic RNA Polymerases
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01:19RNA Structure
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Different Types of RNA Have the Same Basic Structure
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00:43Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...