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Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
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雌激素抑制了血管光滑肌肉细胞依赖的先发性纤维细胞迁移 in vitro.

G Li1, Y F Chen, G L Greene

  • 1University of Alabama at Birmingham, Department of Medicine, Division of Transplantation, Chicago, ILL, USA.

Circulation
|October 12, 1999
PubMed
概括

雌激素通过抑制随机纤维细胞迁移来保护血管损伤. 这通过血管光滑肌细胞上的雌激素受体发生,揭示了一个新的血管保护机制.

科学领域:

  • 血管生物学 血管生物学
  • 内分泌学 在内分泌学.
  • 细胞生物学 细胞生物学

背景情况:

  • 众所周知,雌激素治疗可以防止血管损伤后的neointima形成.
  • 进发性纤维细胞激活和迁移参与了这个过程.
  • 雌激素影响纤维细胞迁移的精确机制需要阐明.

研究的目的:

  • 研究雌激素减弱偶然性纤维细胞迁移的体外机制.
  • 确定血管光滑肌细胞 (VSMC) 在调解雌激素对纤维细胞迁移的影响中的作用.

主要方法:

  • 建立了大鼠血管光滑肌细胞 (VSMC) 和随机纤维细胞的初级培养.
  • 雌激素受体 (ER) 表达的评估是使用RT-PCR和西式涂抹.
  • 纤维细胞迁移量化使用波伊登腔室后VSMC预条件与或没有17β-雌二醇.

主要成果:

  • 发现雌激素受体 (ER) 表达仅限于早期通道VSMCs.
  • 与对照者相比,VSMC受条件的介质显著增加了纤维细胞迁移.
  • 17β-雌激醇治疗VSMC的剂量依赖性抑制纤维细胞迁移.
  • 通过与ER抗剂 (ICI-182780) 的联合治疗,阻断了雌激素的抑制作用.

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结论:

  • 雌激素直接调节控制偶然纤维细胞迁移的因素的VSMC表达.
  • 这种调节通过一种依赖雌激素受体 (ER) 的机制发生.
  • 这些发现表明,雌激素对激素血管保护的新途径.