细胞染色体P450 2C是冠状动脉中的EDHF合成酶
B Fisslthaler1, R Popp, L Kiss
1Institut für Kardiovaskuläre Physiologie, Klinikum der J.W. Goethe-Universität, Frankfurt am Main, Germany.
Nature
|October 16, 1999
概括
一个涉及细胞染色体P450 (CYP) 2C8/34的新途径产生了内皮衍生的高极化因子 (EDHF),对冠状动脉中血管放松至关重要.
科学领域:
- 血管生物学 血管生物学
- 内分泌学 在内分泌学.
- 药理学 药理学 是一个学科.
背景情况:
- 在抑制氧化和环氧酶通路后,内皮依赖血管扩张仍然存在.
- 这种剩余的扩张与内皮衍生的超极化因子 (EDHF) 有关,导致血管光滑肌肉的超极化.
- 精确的EDHF分子身份一直是难以捉摸的.
研究的目的:
- 确定负责猪冠状动脉中EDHF介导放松的分子成分.
- 调查细胞P450 (CYP) 酶在EDHF生产中的作用.
主要方法:
- 在使用β-纳夫托夫拉的猪冠状动脉内皮细胞中诱导CYP 2C8/34.
- 测量了11,12-epoxyeicosatrienoic酸的形成.
- 评估EDHF介导的超极化和放松.
- 在传染冠状动脉中使用反感性寡核酸抑制CYP 2C8/34.
主要成果:
- 诱导CYP 2C8/34增强了11,12-epoxyeicosatrienoic 酸的形成.
- 诱导CYP 2C8/34导致EDHF介导的高极化和放松增加.
- 抑制CYP 2C8/34减弱的EDHF介导的血管反应.
- 一种CYP-epoxygenase产物被确定为EDHF介导放松的重要产物.
结论:
- 细胞染色体P450 (CYP) 2C8/34对于猪冠状动脉中EDHF介导的放松至关重要.
- 在这种血管床中,CYP 2C8/34作为EDHF合成酶起作用.
- 基因CYP-epoxygenase产物是内皮依赖血管扩张的关键媒介.
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