通过BMP对抗的信号继电器控制脊椎动物四肢芽中的SHH/FGF4反循环
A Zúñiga1, A P Haramis, A P McMahon
1Department of Developmental Biology, Faculty of Biology, Utrecht University, The Netherlands.
Nature
|October 19, 1999
概括
格雷姆林,一个骨形态遗传蛋白质对手,从肢体中传递Sonic hedgehog (SHH) 信号.
科学领域:
- 发育生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 脊椎动物的四肢发育依赖于后侧介质体 (偏振区域) 和顶端外皮脊 (AER) 之间的相互作用.
- 声波刺 (SHH) /纤维细胞生长因子4 (FGF4) 反循环对于四肢外生和模式形成至关重要.
研究的目的:
- 阐明SHH信号从后层介质细胞传递到AER的机制.
- 研究格雷姆林在肢体发育过程中调解SHH/FGF4反循环中的作用.
主要方法:
- 来自具有肢体形 (ld) 和Shh.突变的小鼠胚胎的四肢芽的分析.
- 使用格雷姆林表达细胞移植实验到LD突变肢体芽中.
- 对Fgf4,Gremlin和Formin的基因表达分析.
主要成果:
- 介细胞格林林表达对于建立SHH/FGF4反循环至关重要.
- 为了保持格雷姆林和福林表达,需要SHH信号.
- 格雷姆林的形式依赖激活足以诱导Fgf4表达并建立反循环.
结论:
- 格雷姆林作为一个关键的中间体,将SHH信号从后层介质细胞传递到AER.
- 福明-格雷姆林-FGF4级联是肢体模式的关键途径.
- 这项研究揭示了调节脊椎动物四肢发育中的SHH/FGF4反循环的新机制.
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