相关实验视频

Updated: Aug 17, 2026

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Published on: August 11, 2014

沙门氏菌通过表达CD18的细胞通过肠外传播

A Vazquez-Torres¹, J Jones-Carson, A J Bäumler

¹Department of Medicine, University of Colorado Health Sciences Center, Denver 80262, USA.

|November 5, 1999

概括

沙门氏菌通过CD18表达性细胞传播,绕过Peyer补丁M细胞. 这一途径对于对抗胃肠道病原体的全身免疫发展至关重要.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
胃肠病学胃肠病学

背景情况:

皮耶尔贴片中的M细胞是粘膜免疫的关键,也是病原体的入口点.
沙门氏菌Typhimurium入侵需要沙门氏菌病原性岛屿1 (SPI1) 基因才能透M细胞.
缺少SPI1的沙门氏菌以不同的方式传播,这表明了其他系统性传播机制.

研究的目的:

为了研究沙门氏菌从胃肠道传播的机制,独立于M细胞入侵.
确定CD18表达性细胞在沙门氏菌系统传播中的作用.
探索这种CD18依赖性途径对发展系统性免疫力的影响.

主要方法:

在小鼠中口服野生型和SPI1缺乏的沙门氏菌.
在CD18缺乏和野生型小鼠中传播模式的比较.
免疫反应的分析,包括特定的IgG和IgA的产生.

主要成果:

沙门氏菌是通过CD18表达性细胞从肠道运输到血液中.
缺乏CD18的小鼠在口腔感染后对沙门氏菌向肝脏和脏传播具有抵抗力.
用SPI1缺乏的沙门氏菌口服诱导全身IgG反应,而无需刺激粘膜IgA.

结论:

一条依赖CD18的通路促进了沙门氏菌从胃肠道通过肠外传播.
这一途径对沙门氏菌的全身传播至关重要,可能是免疫系统发育的目标.
了解这种路径对于控制胃肠道感染和增强全身免疫力至关重要.

更多相关视频

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Published on: October 25, 2017

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Published on: August 9, 2022

相关概念视频

Amebiasis

Amebiasis

Entamoeba histolytica, a protozoan parasite, is responsible for intestinal and extraintestinal amebiasis. Though a significant proportion of infections remain asymptomatic, approximately 50 million individuals annually are estimated to present with clinical disease, resulting in up to 100,000 deaths globally. The disease burden is disproportionately high in regions with lower socioeconomic status, such as parts of India, Africa, Mexico, and Latin America.Etiology and TransmissionThe infective...