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Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
Published on: March 2, 2014
疫苗病毒的基于动因的运动性模仿受体氨酸激酶信号传递
F Frischknecht1, V Moreau, S Röttger
1Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Nature
|November 30, 1999
概括
疫苗病毒使用基于actin的移动性在细胞之间传播. 这一过程需要病毒蛋白A36R被酸化,然后将Nck和N-WASP招募到actin组装中.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病毒学 病毒学
背景情况:
- 细胞内病原体Listeria monocytogenes和Shigella flexneri利用基于actin的运动性进行细胞间传播.
- 疫苗病毒,类似于这些细菌,也使用基于actin的运动性进行细胞间传播.
- 参与疫苗病毒基于actin的移动性的精确机制和宿主因素在很大程度上仍未被描述.
研究的目的:
- 为了阐明背后的分子机制疫苗病毒基于actin的运动性.
- 为了确定涉及这个过程的特定病毒蛋白和宿主因素.
- 为了了解疫苗病毒如何劫持宿主细胞机械来传播.
主要方法:
- 对病毒蛋白A36R的酸化位点分析.
- 同免疫沉试验用于研究蛋白质相互作用.
- 在酶抑制剂和基因淘汰的存在下对病毒传播的分析.
主要成果:
- 在疫苗病毒蛋白A36R中,Src-家族基因酶对112氨酸的酸化对于基于actin的运动性至关重要.
- 酸化A36R与适应蛋白Nck直接相互作用.
- Nck招募Ena/VASP家族成员N-WASP,以促进活动组合,这对于病毒传播至关重要.
结论:
- 疫苗病毒采用一种信号通路,涉及A36R酸化,Nck和N-WASP以获得基于动因的运动性.
- 病毒模仿宿主细胞的活性蛋白聚合途径,在细胞之间传播.
- 针对这种机制可以提供对控制病毒传播的见解.
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