印记基因的异步复制在雌激细胞中建立并在发育过程中保持
I Simon1, T Tenzen, B E Reubinoff
1Department of Cellular Biochemistry, Hebrew University, Jerusalem, Israel.
Nature
|November 30, 1999
概括
基因组印记涉及异位基因特异性基因表达. 不同的复制时间,建立在雌同体中并维持在整个发育过程中,作为一个表观遗传标记,区分父母的等位基因.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发育生物学 发展生物学
- 基因组学就是基因组学.
背景情况:
- 基因组印记调节了等位基因特异性的基因表达.
- 印记的基因位于大型染色体域中.
- 这些域在S阶段异步复制.
研究的目的:
- 为了研究复制时间和印记基因之间的关联.
- 为了确定何时建立差异复制时间.
- 了解复制时间在表观遗传标记中的作用.
主要方法:
- 光在现场杂交 (FISH) 分析.
- 在S相分化技术中.
- 在各种细胞类型和发育阶段进行分析.
主要成果:
- 不同的复制时间与多种细胞类型的印记基因有关.
- 这种模式在植入前胚胎的早期很明显.
- 复制时间模式是重置在生殖系的游戏生成.
结论:
- 异步复制时间在雌性体中建立.
- 这个时间表在整个开发过程中都保持不变.
- 复制时间可以作为父母等位基因歧视的关键表观遗传标记.
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