血管内皮细胞粘附结合组件和由基-1-酸盐诱导的形态发生
M J Lee1, S Thangada, K P Claffey
1Center for Vascular Biology, Department of Physiology, University of Connecticut Health Center, Farmington 06030-3501, USA.
Cell
|November 11, 1999
概括
脂质的sphingosine-1-phosphate (SPP) 激活了内皮细胞上的特定受体. 这个过程对于形成新的血管至关重要,这是血管生成的一个关键方面.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 血管内皮细胞形成毛细血管网络,以应对血管生成因子.
- 斯芬戈辛-1-酸盐 (SPP) 是一种从血小板中提取的生物活性脂质.
研究的目的:
- 为了研究氨酸-1-酸盐 (SPP) 在调节血管生成中的作用.
- 确定参与SPP介导血管生成的特定受体和信号通路.
主要方法:
- 研究了SPP在内皮细胞上激活EDG-1和EDG-3受体的情况.
- 分析了下游的信号通路,包括Gi/mitogen-activated protein kinase/细胞存活率和Rho/Rac合的附着基结组.
- 评估了EDG-1和EDG-3信号对内皮细胞形态发生的要求.
- 在体内评估了SPP与多血管生长因子的协同效应.
主要成果:
- SPP激活了内皮细胞上的EDG-1和EDG-3 G蛋白结合受体.
- SPP诱导了Gi/mitogen激活蛋白激酶/细胞生存途径.
- SPP促进小GTPaseRho-和Rac-合的粘附结合组.
- EDG-1和EDG-3信号通路对于内皮细胞形成毛细血管状网络至关重要.
- 当与血管生长因子相结合时,SPP在体内增强成熟新血管的形成.
结论:
- 斯芬戈辛-1-酸盐 (SPP) 是一种新型的血管生成调节剂.
- SPP通过EDG-1和EDG-3受体起作用,控制内皮细胞形态发生和新血管形成.
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