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Published on: January 24, 2014
骨髓细胞的自身移植改善了受损的心脏功能
1Department of Surgery, Division of Cardiovascular Surgery, The Toronto Hospital-General Division, University of Toronto, Ontario, Canada.
用5-阿扎丁培养的骨髓细胞 (BMC) 分化为类似心脏的细胞. 移植的BMC改善了心脏功能,并在痕组织中诱导了血管生成.
科学领域:
- 再生医学是一种再生医学.
- 心血管生物学 心血管生物学
- 干细胞疗法是一种干细胞疗法.
背景情况:
- 自主骨髓细胞 (BMCs) 具有心肌修复的潜力.
- 研究BMC分化成心肌细胞对于恢复心脏功能至关重要.
研究的目的:
- 为了评估BMCs的心肌原生差异化.
- 评估心肌瘤痕组织中的BMC存活率和功能影响.
- 为了确定特定培养条件对BMCs的影响.
主要方法:
- 成年大鼠的BMC被培养为5-azacytidine (5-aza),TGFbeta1,或胰岛素.
- 在体内研究涉及将新鲜,培养或经过5aza处理的BMC移植到老鼠心室痕中.
- 心脏功能在移植后8周被评估.
主要成果:
- 只有接受5aza治疗的BMC形成了表达心脏标记物 (热素I,肌酸重链) 的髓管.
- 在BMC移植心脏中观察到心脏样肌肉细胞 in vivo.
- 与对照组相比,接受5aza治疗的BMC移植显著改善了缩和发育压力.
- 所有的BMC移植都在痕组织内促进了血管新生.
结论:
- 用5-aza培养的BMCs在体外和体内都表现出心肌性分化.
- 移植5-aza诱导的BMC可以增强心肌功能,促进血管生成.
- 自异性BMCs代表心脏修复的有前途的治疗策略.
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