相关实验视频
Updated: Jul 19, 2026

25:12
Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain
Published on: July 29, 2007
在活细胞中持续的振荡.
S Danø1, P G Sørensen, F Hynne
1Department of Chemistry and CATS, H.C. Orsted Institute, University of Copenhagen, Denmark. sdd@osc.kiku.dk
Nature
|December 2, 1999
概括
酵母细胞可以使用连续流系统无限期保持持续的糖分溶解振荡. 这种持续的振荡是由Hopf分叉数学描述的,揭示了细胞动态的洞察力.
科学领域:
- 生物化学 生物化学
- 细胞动力学细胞动力学
- 系统生物学 系统生物学
背景情况:
- 从历史上来看,使用短暂的葡萄糖脉冲来研究酵母中的糖溶性振荡.
- 以前的方法专注于测量NADH的振荡,这是一个关键的代谢中间体.
- 了解持续的细胞振荡对于代谢研究至关重要.
研究的目的:
- 建立一种方法来维持酵母细胞在持续的,明确的振荡状态.
- 在数学上描述静止和振荡的代谢行为之间的过渡.
- 为了研究信号分子在酵母糖解振荡中的作用.
主要方法:
- 采用连续流水管系统,为饥饿的酵母细胞提供葡萄糖和化物.
- 观察和分析由此产生的NADH的短暂振荡.
- 应用非线性动力学原理来建模观察到的代谢过渡.
- 进行扰动实验,以评估在振荡期间的细胞合.
主要成果:
- 酵母细胞可以通过持续的营养和废物清除来无限期保持持续的振荡状态.
- 从静止到振荡行为的过渡被Hopf分叉精确地描述.
- 扰动实验表明,在过渡点附近有强大的细胞合,在单个细胞内发生振荡.
- 葡萄糖作为一个信号分子,当葡萄糖载体不和时,它会影响振荡.
结论:
- 一种新的方法使酵母中持续的糖溶解振荡成为可能,从而促进了详细的动力学研究.
- 霍夫分叉为理解代谢状态过渡提供了一个强大的数学框架.
- 细胞合在过渡期附近仍然很强,这表明细胞内机制驱动振荡.
- 葡萄糖和乙甲作为关键信号分子在调节酵母代谢振荡中起作用.
相关概念视频
Non-equilibrium in the Cell
An important concept in studying metabolism and energy is that of chemical equilibrium. Most chemical reactions are reversible. They can proceed in both directions, releasing energy into their environment in one direction, and absorbing it from the environment in the other direction. The same is true for the chemical reactions involved in cell metabolism, such as the breaking down and building up of proteins into and from individual amino acids, respectively. Reactants within a closed system...
Cell Motility through Blebbing
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
Actin Treadmilling
Actin filaments undergo polymerization and depolymerization from either end. The polymerization and depolymerization rates depend on the cytosolic concentration of free G-actins. The polymerization rate is generally higher at the plus or barbed end, while the depolymerization rate is higher at the minus or pointed end. At a steady state, critical concentration describes the concentration of free G-actin monomers at which the polymerization rate at the plus end is equal to that of the...
Cell-matrix's Response to Mechanical Forces
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
Maintenance of the ES Cell State
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Muscle Stimulation Frequency
The contraction strength of muscles is regulated by motor neurons, which modulate the frequency of action potentials dispatched to the motor units based on the body's requirements. This process of varying the muscle stimulation frequency allows muscles to contract with a force that is precisely tailored to the needs of the moment, whether lifting a feather or a heavy box.
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...

