从蚊子,乌和康涅狄格州的库珀中分离出西尼罗病毒
J F Anderson1, T G Andreadis, C R Vossbrinck
1Department of Entomology, Department of Soil and Water, the Connecticut Agricultural Experiment Station, Post Office Box 1106, New Haven, CT 06504, USA. john.f.anderson@po.state.ct.us
Science (New York, N.Y.)
|December 22, 1999
概括
西尼罗病毒 (WNv) 在康涅狄格州的蚊子和鸟类中被检测到. 遗传学分析表明,这些WNV分离物与欧洲菌株密切相关,这表明它们可能在北美建立.
科学领域:
- 兽医病毒学 兽医病毒学
- 亚博病毒学 亚博病毒学 亚博病毒学
- 野生动物疾病生态学 野生动物疾病生态学
背景情况:
- 西尼罗河病毒 (WNv) 是一种蚊子传播的,在非洲,亚洲和欧洲本土存在的弗拉维病毒.
- 这种病毒在鸟类种群中造成了显著的死亡率,并对人类健康构成威胁.
- 以前在北美WNv活动主要记录在美国南部和西部.
研究的目的:
- 为了识别和表征来自康涅狄格州野生动物的西尼罗病毒分离物.
- 确定这些分离物与全球已知的WNV菌株的遗传关系.
- 评估WNv在美国东北部建立的潜在影响.
主要方法:
- 病毒从蚊子池 (Culex pipiens,Aedes vexans) 和鸟类大脑组织 (美国乌,库珀) 中分离.
- 病毒RNA提取和部分基因组测序.
- 使用已确立的WNV基因组序列进行比较的遗传学分析.
主要成果:
- 西尼罗河病毒从康涅狄格州的两种蚊子和两种鸟类中成功分离出来.
- 遗传学分析显示,康涅狄格州的WNV分离物在遗传上彼此相似.
- 这些隔离物与之前在罗马尼亚发现的WNV菌株的关系最为密切,遗传差异分别为2.8%和3.6%.
结论:
- 这些发现表明,康涅狄格州的野生动物中存在西尼罗病毒,可能是从欧洲引入的.
- 与欧洲菌株的遗传相似性表明可能有新的引入途径或持续传播.
- 在北美建立WNv可能会对鸟类和人类产生严重的健康影响.
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