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Updated: Jul 13, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

特尔莫托加海上核糖体循环因子的晶体结构:一个tRNA模仿

M Selmer¹, S Al-Karadaghi, G Hirokawa

¹Molecular Biophysics, Center for Chemistry and Chemical Engineering, Lund University, Post Office Box 124, SE-22100 Lund, Sweden.

Science (New York, N.Y.)

|December 22, 1999

概括

核糖体循环因子 (RRF) 模仿转移RNA (tRNA) 来拆解核糖体后蛋白质合成. 它独特的结构揭示了核糖体循环的新机制,与以前已知的tRNA仿真不同.

科学领域:

分子生物学分子生物学
结构生物学结构生物学
生物化学生物化学

背景情况:

核糖体循环对于蛋白质合成至关重要,涉及核糖体循环因子 (RRF) 和延长因子G (EF-G).
了解RRF功能的结构基础是阐明核糖体循环机制的关键.

研究的目的:

为了确定核糖体循环因子 (RRF) 的晶体结构.
阐明RRF与核糖体复合体相互作用的结构基础.
将RRF的结构与转移RNA (tRNA) 进行比较,以了解其在核糖体循环中的功能.

主要方法:

采用X射线晶体学,以2.55安格斯特罗姆分辨率确定RRF结构.
进行了结构叠加和与转移RNA (tRNA) 的比较分析.

主要成果:

晶体结构显示了RRF的不同寻常的蛋白质折叠,包括三螺旋捆 (域I) 和β/α/β三明治 (域II).
RRF结构与tRNA紧密叠加,仅在3'氨基酸结合端不同.
这种结构模仿表明RRF与tRNA类似地与终结后核糖体复合体结合,促进分解.

结论:

RRF类似tRNA的结构解释了它在核糖体循环中的作用.
观察到的模仿机制是新的,与之前描述的tRNA模仿实例不同.
这些发现为蛋白质合成终止和核糖体再利用的分子机制提供了重要的见解.

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Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

RNA Structure

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

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Different Types of RNA Have the Same Basic Structure
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Transfer RNA Synthesis

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

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