通过基于人口的分子流行病学识别Mycobacterium结核病的W变异爆发
1Public Health Research Institute Tuberculosis Center, Department of Microbiology, New York University School of Medicine, NY 10016, USA.
JAMA
|December 28, 1999
概括
分子类型识别在新泽西州发现了一个以前未被发现的Mycobacterium结核病爆发. 这种使用DNA指纹的方法揭示了不同的W家族菌株和患者人口统计数据,有助于针对性的公共卫生干预.
科学领域:
- 流行病学 流行病学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 传统的监测单独是不足以检测Mycobacterium结核病爆发.
- 分子类型为识别结核病传播提供了更敏感的方法.
- 纽约市M.结核病W菌株的变种以前在新泽西州被发现.
研究的目的:
- 描述新泽西州M.结核病菌株W家族的传播情况.
- 利用分子类型和监测数据来描述菌株传播.
- 确定与W家族菌株相关的特定的人口和地理模式.
主要方法:
- 基于人口的横截面研究分析了1996年1月至1998年9月的结核病例.
- 分子类型技术包括IS6110DNA指纹,PGRS类型,spoligotyping和VNTR分析.
- 菌株特征是用常规监测数据补充的.
主要成果:
- 在1207个分离物中,68个 (6%) 属于W族. 根据IS6110模式确定了两个不同的组 (A和B).
- 组A菌株 (43名患者) 关系密切,而组B菌株 (25名患者) 更为多样化.
- 组A患者主要是美国出生的,黑人,艾滋病毒阳性,居住在新泽西东北部的城市地区;组B患者主要是分散在全州的非美国出生的亚洲人.
结论:
- 多种分子技术与监测数据相结合,成功地发现了以前未被发现的结核病爆发.
- 疫情涉及W家族遗传系的一个明显分支.
- 分子菌株类型化为启动疫情调查提供了一个积极的策略,增强了传统的监测.
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