Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Removal of Cd, Cu, Pb, and Zn from aqueous solutions by biochars.

Environmental science and pollution research international·2015
Same author

Importance of salt fingering for new nitrogen supply in the oligotrophic ocean.

Nature communications·2015
Same author

Search for Resonances Decaying to Top and Bottom Quarks with the CDF Experiment.

Physical review letters·2015
Same author

Vemurafenib pharmacokinetics and its correlation with efficacy and safety in outpatients with advanced BRAF-mutated melanoma.

Targeted oncology·2015
Same author

Tevatron constraints on models of the Higgs boson with exotic spin and parity using decays to bottom-antibottom quark pairs.

Physical review letters·2015
Same author

Constraints on models of the Higgs boson with exotic spin and parity using decays to bottom-antibottom quarks in the full CDF data set.

Physical review letters·2015

相关实验视频

Updated: Jul 12, 2026

Antibody Staining in C. Elegans Using "Freeze-Cracking"
13:10

Antibody Staining in C. Elegans Using "Freeze-Cracking"

Published on: October 14, 2013

在C. elegans中绘制蛋白相互作用图,使用参与发育的蛋白质.

A J Walhout1, R Sordella, X Lu

  • 1Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA.

Science (New York, N.Y.)
|December 30, 1999
PubMed
概括

在Caenorhabditis elegans中使用酵母二混合分析进行大规模蛋白质相互作用映射,成功注释了未表征的基因产物. 这种方法在全基因组范围内是可行的,有助于理解分子机制和人类疾病.

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 大规模的蛋白质相互作用映射对于从完整基因组序列中功能性注释蛋白质至关重要.
  • 酵母双混合系统是识别蛋白质-蛋白质相互作用的强大方法.
  • 凯诺哈比迪斯提供了一个有价值的模型生物体,用于研究基本的生物过程.

研究的目的:

  • 为了检查大规模蛋白质相互作用绘制的可行性,使用酵母两杂交分析在Caenorhabditis elegans.
  • 为了功能性地注释参与阴道发育的未经表征的基因产物.
  • 为了生成C. elegans蛋白质的一个子集的蛋白质相互作用图.

主要方法:

  • 利用酵母双混合系统进行高通量蛋白相互作用查.
  • 专注于27种已知参与Caenorhabditis elegans vulval发育的蛋白质.
  • 根据实验结果构建了一个蛋白质相互作用地图.

主要成果:

  • 蛋白质相互作用地图揭示了以前已知的和新的潜在蛋白质相互作用.
  • 成功为大约100个未表征的基因产物提供了功能注释.
  • 证明了在C. elegans中全基因组蛋白相互作用映射项目的可行性.

更多相关视频

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining
06:04

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

Published on: September 16, 2022

相关实验视频

Last Updated: Jul 12, 2026

Antibody Staining in C. Elegans Using "Freeze-Cracking"
13:10

Antibody Staining in C. Elegans Using "Freeze-Cracking"

Published on: October 14, 2013

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining
06:04

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

Published on: September 16, 2022

结论:

  • 大规模的蛋白质相互作用映射是Caenorhabditis elegans.功能基因组学的一个可行的策略.
  • 生成的相互作用地图为C. elegans.中的分子机制提供了洞察力.
  • 这种方法对通过保存的分子通路来理解人类疾病有潜在的影响.