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在缺乏生物钟的水缺陷小鼠中,mPer1和mPer2的光学诱导

H Okamura¹, S Miyake, Y Sumi

¹Department of Anatomy and Brain Science, Kobe University School of Medicine, Kobe 650-0017, Japan. okamurah@kobe-u.ac.jp

Science (New York, N.Y.)

|January 5, 2000

概括

缺少两种哺乳动物密码染色体 (mCRY1和mCRY2) 的小鼠失去了生物钟,导致心律失常行为. 然而,这些加密染色体对于光诱导的时钟重置并不是必不可少的.

科学领域:

时间生物学时间生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

循环节律是由涉及时钟基因的分子反循环调节的.
哺乳动物的密码染色体 (mCRY1和mCRY2) 是昼夜时钟的关键组成部分.
需要进一步阐明mCRY1和mCRY2在反循环负端的确切作用.

研究的目的:

研究mCRY1和mCRY2在维持昼夜节律的功能.
确定mCRY1和mCRY2在昼夜反循环的负端中的作用.
评估mCRY1和mCRY2对于光感应相位移的必要性.

主要方法:

对mCry1和mCry2双突变小鼠的生成和行为分析.
对mPer1和mPer2mRNA表达在上神核和外围组织中的定量分析.
在突变小鼠中对光诱导的相位转移反应的评估.

主要成果:

缺乏mCRY1和mCRY2的小鼠表现出行为失常.
废除了mPer1和mPer2的循环表达,具有构成性高的mRNA水平.
发现mCRY1和mCRY2对于光引起的相位转移是没有必要的.

结论:

哺乳动物的CRY蛋白 (mCRY1和mCRY2) 对于生物钟的运行至关重要.
mCRY1和mCRY2在昼夜反循环的负分支中起作用.
昼夜时钟的光感应相位转移不需要mCRY1和mCRY2.

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