对于正确的3'拼接位选择,需要RNA拼接因子hSlu7
1Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Nature
|January 26, 2000
概括
拼接因子hSlu7通过正确地保持第1个特异体,确保精确的信使RNA (mRNA) 拼接. 没有hSlu7,外因子1被松散地保持,导致在拼接过程中错误的AG二核酸选择.
科学领域:
- 分子生物学分子生物学
- RNA拼接机制的机制
背景情况:
- 使者RNA (mRNA) 拼接涉及两个催化步骤,以产生成熟的mRNA.
- 拼接的II步骤要求外子1攻击3'拼接部位的腺-瓜 (AG) 二核酸.
- 从多个下游选项中选择正确的AG二核酸是不完全理解的.
研究的目的:
- 识别和描述一个对正确的AG二核酸选择负责的甲基动物剪接因子.
- 阐明在拼接过程中区分正确的AG的机制.
主要方法:
- 分析了在没有发现的拼接因子 (hSlu7) 的情况下组装的拼接体.
- 在体外研究AG选择的忠实性.
主要成果:
- 鉴定出一种甲基动物拼接因子hSlu7,它对于正确的AG选择至关重要.
- 在没有hSlu7的情况下,正确的AG被抑制使用,错误的AG被激活.
- 异位基因1与缺少hSlu7的结合基因松散相关,阻止了对正确的AG的精确准.
结论:
- 通过确保正确的AG二核酸被准,hSlu7对于保持mRNA拼接的忠实性至关重要.
- 蛋白质hSlu7的功能是通过在结合体内紧紧地住异构1来,使预先指定的AG受到准确的攻击.
相关概念视频
RNA Splicing
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RNA Splicing
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Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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