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相关概念视频

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

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在中性粒细胞化学毒性作用期间,化学吸引剂受体信号的极化.

G Servant1, O D Weiner, P Herzmark

  • 1Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94143, USA.

Science (New York, N.Y.)
|February 11, 2000
PubMed
概括

哺乳动物细胞极性对于定向运动 (化学反应) 至关重要,由内部信号梯度调节. 一个探测器显示这些梯度比外部信号更,并且取决于Rho GTPase活动.

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • 在哺乳动物细胞中,形态极性对于有针对性的细胞运动 (称为化学反应) 是必不可少的.
  • 了解建立和维持这种极性的细胞内信号是理解细胞迁移的关键.
  • AKT蛋白质激酶 (也称为蛋白质激酶B) 的pleckstrin同质 (PH) 域是已知的酸丁酸 (3,4,5) -三酸盐 (PIP3) 的传感器,是一种参与细胞信号传递的脂质第二信使.

研究的目的:

  • 为了研究细胞内信号事件,建立和保持在化学反应期间的形态极性.
  • 为了可视化和量化细胞内信号的空间分布,以响应化学吸引剂.
  • 为了确定参与生成这些内部信号梯度的分子参与者.

主要方法:

  • 光标记探针的表达,在中性粒细胞中,与绿色光蛋白 (PHAKT-GFP) 合的AKT蛋白激酶的斑块蛋白同质域.
  • 用化学吸引剂刺激中性粒细胞,并使用光显微镜实时观察PHAKT-GFP局部化.
  • 使用来自Clostridium difficile的毒素-B抑制PHAKT-GFP转位.

主要成果:

  • 在化学吸引剂暴露后,PHAKT-GFP被选择性地招募到中性粒细胞前端的细胞膜中.
  • 观察到的招募表明,内部信号梯度的形成明显比外部化学吸引剂梯度更.

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  • 通过毒素-B抑制PHAKT-GFP转位证明了对于这个过程需要Rho瓜诺辛三酸酶 (GTPase) 活性.

    • 结论:

    • 通过PHAKT-GFP可视化的细胞内信号梯度在化疗过程中建立,并且比化学吸引剂的外部梯度更.
    • 罗GTPase活性对于产生和维持这些的细胞内信号梯度至关重要,这些梯度驱动细胞极性.
    • 这些发现提供了关于哺乳动物细胞中指向细胞迁移和极性背后的分子机制的见解.