关于稳定选择在真核细胞增强元件中的证据
M Z Ludwig1, C Bergman, N H Patel
1Department of Ecology and Evolution, University of Chicago, Illinois 60637, USA. mludwig@midway.uchicago.edu
Nature
|February 17, 2000
概括
基因调节元件的进化变化,如增强剂,可以产生功能后果. 这项研究表明,Drosophila增强剂的序列差异被共同进化的变化掩盖,影响基因表达模式.
科学领域:
- 发育生物学是发展生物学.
- 进化遗传学的进化遗传学
- 分子生物学分子生物学
背景情况:
- 细胞基因表达依赖于由转录因子结合的 cis 调节模块 (增强剂).
- 德洛索菲拉 (Drosophila melanogaster) 中的均跳过的条纹2元素是一种经过充分研究的增强剂,可以控制特定的基因表达模式.
- 尽管保留了表达,但增强器序列仍在进化,呈现出一种进化悖论.
研究的目的:
- 为了研究在偶跳过的条纹2增强器中的序列分歧的功能影响.
- 了解增强剂的进化变化如何影响基因调节.
- 为真核生物调节序列的进化提出一个模型.
主要方法:
- 通过交换来自不同物种的本地条纹2元素的半部分来构建嵌合体增强剂.
- 评估Drosophila胚胎中由这些嵌合体增强剂驱动的记者基因表达.
- 跨物种增强元件的比较序列分析.
主要成果:
- 通过将不同物种的两半组合而形成的化学增强剂,未能在野生类型模式中驱动记者基因表达.
- 物种增强剂之间的序列差异对基因表达有功能影响.
- 这些功能后果似乎被其他共同进化的序列修改所掩盖.
结论:
- 增强剂序列的进化变化确实对基因调节有功能影响.
- 同进化过程可以掩盖单个序列变化的功能影响.
- 基于这些发现,提出了真核生物调节序列进化的模型.
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