子调节的 Gli3 处理在发育中的脊椎动物四肢中产生前/后抑制剂梯度
B Wang1, J F Fallon, P A Beachy
1Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Cell
|February 29, 2000
概括
刺信号通过 Gli3 蛋白质处理来调节四肢发育. 这种处理产生了Gli3的抑制器形式,对于建立前后模式至关重要,并与Sonic hedgehog对抗.
科学领域:
- 发育生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- Ci/Gli 指蛋白是 Hedgehog (Hh) 信号传递的关键媒介.
- 在Drosophila中,Ci的转录作用是由Hh调节的,PKA依赖的蛋白质分解处理决定的.
- 脊椎动物的四肢发育依赖于发育信号通路的精确空间和时间调节.
研究的目的:
- 研究脊椎动物 Gli3 处理在肢体发育中的作用和调节.
- 了解 Gli3 处理如何促进前后模式.
- 探索 Gli3 调节错误在遗传综合征和发育突变的后果.
主要方法:
- 在成长中的四肢中分析脊椎动物Gli3的PKA依赖性处理.
- 研究Sonic hedgehog (Shh) 蛋白在Gli3处理中的对抗作用.
- 对人类遗传综合征中的Gli3突变和talpid2突变的检查.
主要成果:
- 基3的PKA依赖性处理产生了一个强大的抑制形式.
- 这种Gli3抑制剂梯度是由后部局部化的Sonic hedgehog. antagonistically建立的.
- 在Gli3处理或突变中出现的干扰会导致肢体模式形.
结论:
- 在成长中的四肢中, Gli3 处理对于建立前后极性至关重要.
- Gli3抑制器功能专门用于负调节子通路.
- Gli3 处理的失调有助于先天性四肢形.
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