Ras1促进了Drosophila翅膀中的细胞生长
1Molecular and Cellular Biology Program, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Cell
|February 29, 2000
概括
拉斯GTPase信号控制细胞增殖通过调节细胞生长和G1/S阶段过渡. 激活Ras会促进生长和细胞循环的进展,而非激活Ras会阻碍Drosophila的生长并引起细胞亡.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 拉斯GTPase是一个关键的调节器,将细胞外信号与细胞增殖联系起来.
- 在体内了解Ras的功能对于破译生长控制机制至关重要.
研究的目的:
- 研究Ras在Drosophila中调节细胞增殖中的体内作用.
- 阐明Ras信号的下游效应因子和细胞周期目标.
主要方法:
- 在正在发育的Drosophila翅膀中的特定细胞克隆中Ras的激活和非激活.
- 细胞大小,生长速度,细胞周期进展 (G1,G2/M) 和细胞亡的分析.
- 评估关键的调节分子,包括dMyc和环林E水平.
主要成果:
- 拉斯无活化导致细胞大小减少,生长缓慢,G1细胞周期停止,通过细胞竞争导致细胞亡.
- 拉斯激活增加了细胞大小,生长率,并促进了G1/S阶段过渡.
- 拉斯及其下游效应剂dMyc在转录后上调了环林E水平.
- 拉斯介导增长增强剂没有扰乱G2/M进展或正常细胞周期的退出.
结论:
- 拉斯主要的功能是促进细胞生长,这与通过环林E的G1/S阶段进展密切相关.
- 拉斯信号传递对于维持细胞生长和在发育过程中生存至关重要.
- 拉斯介导的生长调节与其对后期细胞周期阶段和发育细胞周期的作用不同.
相关概念视频
02:36Cells Coordinate Growth and Proliferation
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...