需要因诺三酸盐受体来激活商店运行的Ca2+通道
H T Ma1, R L Patterson, D B van Rossum
1Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.
Science (New York, N.Y.)
|March 4, 2000
概括
伊诺西三酸盐受体 (InsP3R) 对于信号传递至关重要,它通过将内分泌网膜 (ER) 的储存与血膜通道连接起来. 这项研究阐明了InsP3RR.
科学领域:
- 细胞生物学 细胞生物学
- 分子生理学分子生理学
- 信号传递 信号传递
背景情况:
- 连接内细胞网膜 (ER) 储存与血膜 (PM) 储存运行的通道 (SOCs) 的机制对于信号传输至关重要,但人们对其了解甚少.
- SOCs可能与TRP通道家族有关,但它们的确切关系和激活机制尚不清楚.
研究的目的:
- 研究ER Ca2+储存和PM SOC之间的合机制.
- 将内源性SOC与TRP3通道的特性进行比较,并阐明它们的激活要求.
- 确定伊诺西三酸盐受体 (InsP3Rs) 在SOC和TRP通道功能和ER-PM合中的作用.
主要方法:
- 在人类胚胎脏 (HEK293) 细胞中比较内源性SOC和稳定表达的TRP3通道.
- 在缩的皮质F-actin.in存在时评估通道激活.
- 使用2-aminoethoxydiphenyl酸盐,一个细胞透的InsP3R抑制剂,以阻止通道活性.
主要成果:
- 发现TRP3道与SOC不同,是独立于商店的.
- 凝聚的皮质F-actin抑制了SOC和TRP3通道的激活,这表明ER-PM相互作用对合至关重要.
- 通过2-aminoethoxydiphenyl borate抑制InsP3R功能,阻断了受体诱导的TRP3激活和储存诱导的SOC激活.
结论:
- 在SOC和TRP道的开放中,InsP3R在中介方面发挥着至关重要的作用.
- 伊诺西三酸盐受体对于维持ER存储耗尽和SOC激活之间的生理合至关重要.
- 由InsP3Rs介导的ER-PM相互作用对信号传递中的SOC和TRP通道功能至关重要.
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