这种LIM homeobox基因Lhx9对于小鼠的生殖腺形成至关重要
O S Birk1, D E Casiano, C A Wassif
1Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA.
Nature
|March 8, 2000
概括
在小鼠中,LIM homeobox 基因 Lhx9 对于生殖腺发育至关重要. 它的缺失阻止了生殖腺的形成,导致雄性小鼠发展为雌性,并表明它在人类生殖腺生成中发挥了作用.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 生殖生物学 生殖生物学
背景情况:
- 哺乳动物的生殖腺 (卵巢和丸) 从泌尿器官脊脊形成.
- 性别决定涉及丸产生的丸激素和抗穆勒尔激素等激素.
- LIM家庭盒基因Lhx9在胚胎发育中起作用.
研究的目的:
- 研究Lhx9基因在哺乳动物性腺发育中的功能.
- 确定Lhx9在生殖腺形成和分化中的作用.
- 为了探索Lhx9突变与人类淋巴腺发育之间的潜在联系.
主要方法:
- 在胚胎发育期间研究了小鼠泌尿器官脊柱中的Lhx9基因表达.
- 产生并分析了缺乏功能Lhx9.9的小鼠.
- 评估Lhx9缺陷胚胎中的生殖细胞迁移,体细胞增殖和性腺形成.
- 在Lhx9突变体中检查了类固醇生成因子1 (Sf1) 的表达.
主要成果:
- Lhx9转录物存在于早期的泌尿器官脊柱,后来在发生性腺发育的间歇性区域.
- 缺少Lhx9的小鼠表现出正常的生殖细胞迁移,但由于缺乏体细胞增殖,未能形成离散的生殖腺.
- 遗传上雄性Lhx9缺乏的小鼠是表型上雌性,缺乏丸激素和抗穆勒激素.
- 关键性淋巴发育基因Sf1的表达在Lhx9缺陷的生殖器脊柱中显著降低.
- Lhx9突变体没有表现出其他重大发育异常.
结论:
- Lhx9对于体细胞的增殖和生殖腺的形成至关重要.
- 在发育级联中,Lhx9在Sf1的上游作用,调节淋巴发生.
- 在人类中,Lhx9的突变可能是隔离性淋巴腺发生的原因.
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