在Brassica stigma中,S受体激酶决定了自我不相容性
T Takasaki1, K Hatakeyama, G Suzuki
1Research Institute of Seed Production Co., Ltd., Sendai, Japan. taka@kobe-u.ac.jp
Nature
|March 8, 2000
概括
布拉西卡的自我不相容性依赖于S受体激酶 (SRK) 和S位点糖蛋白 (SLG) 基因. 单独的SRK决定了花粉排斥的特异性,而SLG则增强了自我不相容性反应.
科学领域:
- 植物生殖生物学 植物生殖生物学
- 分子遗传学 分子遗传学
- 植物育种 植物育种
背景情况:
- 自我不相容性 (SI) 是布拉西卡的关键生殖障碍,防止自我受精.
- 控制SI的S位点涉及两个关键基因:S受体激酶 (SRK) 和S位点糖蛋白 (SLG).
- 这些基因表达在斑点 papillar 细胞中,并被认为可以调解花粉的识别和排斥.
研究的目的:
- 阐明SRK和SLG在Brassica自我不相容性中的个别作用.
- 为了确定单独的SRK或SLG是否可以赋予自我花粉排斥.
- 调查SRK和SLG对自我不相容反应的协同效应.
主要方法:
- 用SRK28和SLG28转基因对自我不兼容的Brassica rapa植物进行基因转化.
- 在单独表达SRK28,单独表达SLG28或两种转基因植物中的花粉排斥的分析.
- 修饰布拉西卡植物自我不相容反应的表型评估.
主要成果:
- 单独对SRK28转基因的表达赋予了拒绝自我 (S28) 花粉的能力.
- 单靠SLG28转基因表达并没有产生自我花粉排斥.
- 由SRK28介导的花粉排斥能力因SLG28.8的共同表达而显著增强.
结论:
- 仅SRK就足以确定Brassica自我不相容性中标记的S哈普类型特异性.
- SLG作为调节器起作用,增强自我不兼容性反应的充分表现.
- 这些发现澄清了SRK和SLG在Brassica生殖隔离中的不同但合作作用.
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