在Drosophila中的候选味觉受体
P J Clyne1, C G Warr, J R Carlson
1Department of Molecular, Cellular, and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520-8103, USA.
概括
研究人员在Drosophila中发现了一个由七个跨膜域蛋白组成的大家族,其中18个基因在标签体中表达,这表明它们可能作为味觉感知的味觉受体起作用.
科学领域:
- 分子生物学分子生物学
- 神经科学是一个神经科学.
- 遗传学 遗传学 是一个
背景情况:
- 动物的味觉感知和初始味道信号事件的分子机制在很大程度上是未知的.
- 七个跨膜域蛋白质代表着一个多样化的蛋白质家族,在感官感知中发挥着已知的作用.
研究的目的:
- 为了确定Drosophila.中的潜在的新型味觉受体.
- 为了研究一种新发现的蛋白家族在味觉器官中的表达模式.
主要方法:
- 对Drosophila基因组的生物信息分析以确定七种跨膜域蛋白质.
- 使用定量PCR和现场杂交的基因表达分析.
- 在缺乏味觉神经元的Drosophila突变体中检查基因表达 (天花神经70).
主要成果:
- 鉴定出一个由19个7个跨膜域蛋白组成的庞大而多样化的家族.
- 在这19个基因中,有18个在Drosophila labellum中表现出特定的表达,Drosophila labellum是主要的味觉器官.
- 这些基因的表达在其他组织和天花-neuro70突变的标签体中不存在.
结论:
- 根据结构相似性和味觉器官的组织特异表达,鉴定的蛋白质家族可能编码了一个不同的味觉受体家族.
- 这些发现为了解Drosophila.味觉感知分子基础提供了基础.
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