鉴定DC-SIGN,一种新型树突细胞特异性ICAM-3受体,它支持初级免疫反应
T B Geijtenbeek1, R Torensma, S J van Vliet
1Department of Tumor Immunology, University Medical Center St. Radboud, Nijmegen, The Netherlands.
Cell
|March 18, 2000
概括
休息的T细胞使用ICAM-3最初与树突细胞 (DC) 接触. 一种新的DC受体,DC-SIGN,与ICAM-3结合,调解T细胞粘附和增殖,这对免疫反应至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 启动初级免疫反应需要树突细胞 (DC) 和休息T细胞之间的接触.
- 细胞间粘附分子 (ICAMs) 在免疫系统内的细胞相互作用中起着关键作用.
研究的目的:
- 研究ICAM-3在与DCs初次接触时对静止T细胞的作用.
- 为了确定负责结合ICAM-3和调解T细胞粘附的特定DC受体.
主要方法:
- 利用DC-T细胞相互作用的体外和体外模型.
- 采用基于抗体的抑制试验来评估DC-SIGN的功能作用.
- 研究了ICAM-3和DC-SIGN之间的结合亲和关系.
主要成果:
- 鉴定了DC-SIGN,一种在DC上高度表达的C型乳素,作为ICAM-3的高亲和度受体.
- 证明DC-SIGN在T细胞和DC之间调解了短暂的粘附.
- 表明阻断DC-SIGN的抗体抑制了休息T细胞的DC诱导的增殖.
结论:
- DC-SIGN是T细胞和DCs之间初始接触的关键调解者.
- 对ICAM-3的DC-SIGN结合稳定了DC-T细胞结合体,促进了T细胞受体的参与和随后的T细胞激活.
- 这些发现凸显了DC-SIGN在启动适应性免疫反应中的关键作用.
更多相关视频
相关概念视频
01:25Selectins
Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
01:31Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...