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大肠杆菌原酶RNA聚合酶域的结构

J L Keck¹, D D Roche, A S Lynch

¹Department of Molecular and Cell Biology, University of California, Berkeley, 229 Stanley Hall, no. 3206, Berkeley, CA 94720, USA.

Science (New York, N.Y.)

|March 31, 2000

概括

研究人员确定了大肠杆菌DnaG原酶的晶体结构. 这种酶合成了DNA复制必不可少的RNA原始酶,揭示了独特的活性站点架构.

科学领域:

结构生物学结构生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

细胞生物依赖原酶,一种RNA聚合酶,以创建RNA原始酶.
这些RNA原体对于启动DNA复制至关重要.
大肠杆菌DnaG蛋白是参与DNA复制的关键原酶.

研究的目的:

为了确定大肠杆菌DnaG原酶的催化核的高分辨率晶体结构.
阐明主动站点架构及其与其他聚合物酶的关系.
了解DnaG在复杂体内的功能结构的基础.

主要方法:

高分辨率的晶体结构确定大肠杆菌DnaG原酶催化核.

主要成果:

确定的结构揭示了与其他DNA或RNA聚合酶手掌折叠区别的活性位点架构.
DnaG结构表现出与顶端折叠的关系.
结构表明,在复合体内,核酸结合和定位的不变残留物存在一个槽.

结论:

DnaG原酶具有独特的活性位点折叠,与toprim折叠相关,以前在其他聚合酶中没有见过.
结构数据表明DnaG可能如何结合核酸并与其他复制机械组件相互作用.
DnaG被定位为接受单链DNA,直接来自复制体内的复制酶.

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