Gcn5 原蛋白协调核细胞重塑
P Syntichaki1, I Topalidou, G Thireos
1Institute of Molecular Biology and Biotechnology, FORTH, and Department of Biology, University of Crete, Heraklion, Greece.
Nature
|April 4, 2000
概括
基因组乙转移酶 (HATs) 的基因组链接基因组乙化与核细胞重塑. 这种相互作用对于Swi2依赖的转录激活至关重要,稳定了Swi/Snf复合体.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因规则 基因规则
背景情况:
- 转录因子对真核突变促进体的获取需要通过多蛋白质复合体对染色质结构进行修改.
- 基因组乙转移酶 (HAT) 和ATP酶 (如Swi2在Swi/Snf中) 是重塑复合物的关键类别.
- 在发起人监管中,HATs和Swi/Snf复合体之间的功能联系仍然不清楚.
研究的目的:
- 为了研究原体的作用,一个保存的HAT模块,在将基因素乙化与随后的核细胞重塑联系起来.
- 为了确定多胺-乙化氨酸结合是否在体内对于HAT活性和下游事件至关重要.
主要方法:
- 利用一种特定的促进器系统,研究Gcn5原蛋白的体内功能要求.
- 评估了原蛋白残留的必要性,这些残留对体外乙-氨酸结合至关重要,用于Gcn5介导的基因素乙化.
- 评估了基基因突变对Swi2依赖核细胞重塑和转录激活的影响.
主要成果:
- 对于体外乙-氨酸结合必不可少的基因残留物,对于体内Gcn5介导的基因素乙化是不可或缺的.
- 这些特定的原体残留物对于随后的Swi2依赖核细胞重塑和转录激活至关重要.
- 已经证明,Gcn5原蛋白能够稳定促进体中的Swi/Snf复合体.
结论:
- 基基因作为基因素乙化和核细胞重塑机制之间的关键环节.
- 基原-乙-氨酸相互作用对于准和稳定Swi/Snf复合体至关重要,促进转录激活.
- 这项研究阐明了真核生物基因表达的协调调节的一个关键机制.
相关概念视频
02:33The Nucleosome
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
02:10The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
02:54Nucleosome Remodeling
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
01:12The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...