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Published on: March 16, 2016
S15,S6,S18-rRNA复合体的结构:30S核糖体中心域的组合
S C Agalarov1, G Sridhar Prasad, P M Funke
1Department of Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. dave@scripps.edu
概括
一个Thermus thermophilus 30S核糖体子单元复合物的结构揭示了蛋白质S15如何重组RNA,从而使核糖体蛋白质S6和S18的组装成为可能. 这种S15诱导的RNA折叠对核糖体生物生成至关重要.
科学领域:
- 结构生物学 结构生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 核糖体生物发生是一个复杂的过程,涉及核糖体RNA和蛋白质的精确组装.
- 30S核糖体子单元在蛋白质合成中起着至关重要的作用.
- 了解早期组装步骤的结构基础是破译核糖体形成的关键.
研究的目的:
- 为了确定来自Thermus thermophilus 30S核糖体子单元的关键核糖核蛋白复合物的高分辨率晶体结构.
- 阐明核糖体蛋白S15在早期组装期间核糖体RNA的结构组织中的作用.
- 提供对核糖体蛋白质S6和S18的顺序结合的见解.
主要方法:
- 使用X射线晶体学来解决晶体结构.
- 该结构以2.6安格斯特罗姆的原子分辨率确定.
- 进行了RNA-蛋白相互作用的生物化学分析.
主要成果:
- 阐明了由104核酸RNA片段和S15,S6和S18核糖体蛋白组成的70千多复合体的晶体结构.
- 观察到蛋白质S15在组装早期与核糖体RNA结合,从而诱导两个三螺旋结的关键形状重组.
- 这种S15介导的RNA折叠对于随后的核糖体蛋白S6和S18的招募和结合至关重要.
结论:
- 该结构突出了蛋白质S15在通过RNA结构调制启动核糖体组装中的关键作用.
- 中央域RNA的S15诱导的重组是其他核糖体蛋白质正确组装的先决条件.
- 这项研究为了解30S核糖体子单元形成的复杂过程提供了结构基础.
相关概念视频
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Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

