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相关概念视频

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

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相关实验视频

Updated: Jun 30, 2026

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

在S相检查点路径中的ATM酸化物p95/nbs1

D S Lim1, S T Kim, B Xu

  • 1Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.

Nature
|April 15, 2000
PubMed
概括
此摘要是机器生成的。

过敏性-脑膜炎症 (AT) 和尼米根断裂综合征 (NBS) 具有相同的辐射敏感性. 在NBS细胞中的ATM激酶激活和ATM依赖的p95/nbs1酸化将这些蛋白质连接在一个共同的DNA损伤反应途径中.

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Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

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相关实验视频

Last Updated: Jun 30, 2026

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 缺血症和尼姆根断裂综合征 (NBS) 是罕见的疾病,具有共同的症状,如染色体不稳定性和辐射敏感性.
  • ATM (AT) 和p95/nbs1 (NBS) 基因的突变是这些条件的基础,影响DNA损伤反应途径.

研究的目的:

  • 为了调查ATM和p95/nbs1之间的功能关系,由于AT和NBS之间观察到的相似之处.
  • 阐明p95/nbs1在细胞对电离辐射反应中的ATM依赖酸化的作用.

主要方法:

  • 评估在电离辐射后NBS细胞中的ATM激酶激活和ATM依赖反应.
  • 评估p95/nbs1在血清343.3中的体外和体内酸化.
  • 使用突变的p95/nbs1构造 (非酸化) 来检查S相检查点功能.

主要成果:

  • 电离辐射激活了ATM激酶,并诱导了NBS细胞中的ATM依赖反应,这表明p95/nbs1对于辐射后的ATM信号不是必不可少的.
  • 在电离辐射后,p95/nbs1经过了ATM依赖的酸化在343级.
  • 一个突变的p95/nbs1构造缺少ATM化部位,破坏了正常细胞中的S相检查点,并未能挽救NBS细胞中的缺陷.

结论:

  • ATM和p95/nbs1在一个共同的信号通路中功能地联系在一起.
  • 对于对电离辐射的S相检查点反应,p95/nbs1的ATM依赖酸化至关重要,这解释了AT和NBS之间的表型重叠.