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Isolation of Leukocytes from the Human Maternal-fetal Interface
Published on: May 21, 2015
人类淋巴细胞中安纳米德酸酶度降低与流产之间的关系
M Maccarrone1, H Valensise, M Bari
1Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Italy.
Lancet (London, England)
|April 25, 2000
概括
孕妇的低胺酶活性是自发流产的早期标志物. 这一发现可能会导致新的诊断工具,用于监测早期怀孕和预防流产.
科学领域:
- 生殖生物学 生殖生物学
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
背景情况:
- 安纳米德等内分类大麻素可能会对怀孕和胚胎发育产生负面影响.
- 胺酸酸酶活性对于调节胺水平至关重要,有可能预防怀孕失败和不孕.
研究的目的:
- 为了研究一种假设,即安纳米德酸酶活性对于预防怀孕失败至关重要.
- 为了确定安纳米德酸酶活性是否可以预测孕妇流产.
主要方法:
- 通过放射性染色学测量了50名孕妇 (妊娠6~11周) 的外周淋巴细胞中的安纳胺酸酶活性.
- 用ELISA评估了蛋白质水平上的酶表达.
- 第二项研究测量了120名孕妇 (7-8周怀孕) 的安纳米德酸盐度,并将其与怀孕结果相关联.
主要成果:
- 流产的女性与成功怀孕的女性相比,安纳米德酸酶活性显著降低.
- 一个特定的度胺酸酶度 (ELISA光密度为0.15在450nm) 的值有效地区分了流产和没有流产的女性.
- 在第二项研究中,所有15名安纳米德酶度低的女性都流产,而高度组中只有一名女性流产.
结论:
- 淋巴细胞中安纳米德酸酶活性和表达的减少是自发流产的早期标志物 (妊娠8周以下).
- 这种酶标记物可能对大规模的,常规的怀孕监测和诊断有价值.
- 内分泌大麻素似乎对调节淋巴细胞依赖的细胞因子网络至关重要,这对于生育和成功怀孕至关重要.
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