相关实验视频

Updated: May 18, 2026

Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons
10:13

Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons

Published on: February 10, 2016

直接观察由Arp2/3复合体和WASP/Scar蛋白质组成的树突性actin丝网

L Blanchoin1, K J Amann, H N Higgs

  • 1Structural Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA.

Nature
|May 9, 2000
PubMed
概括

对于细胞爬行至关重要的Arp2/3复合体,在核化过程中形成分支的活性蛋白网络,而不是之后. 这个过程由WASP/Scar蛋白,封闭蛋白和profilin调节,使有效的actin网络组装和拆卸成为可能.

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Actin Polymerization01:42

Actin Polymerization

Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
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Generation of Straight or Branched Actin Filaments

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Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
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Actin Filament Depolymerization

Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
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Formation of Higher-order Actin Filaments

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The high-order actin networks...
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