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在自身免疫性肝炎中识别SLA/LP自身抗体的抗原

I Wies¹, S Brunner, J Henninger

¹Department of Medicine, Johannes Gutenberg University, Mainz, Germany.

Lancet (London, England)

|May 9, 2000

概括

研究人员确定了自身免疫性肝炎中可溶性肝抗原/肝胰腺 (SLA/LP) 自体抗体的主要向抗原. 这一发现使可靠的诊断分析和进一步研究疾病成为可能.

科学领域:

免疫学免疫学免疫学
肝病学肝病学是一种肝病学.
分子生物学分子生物学

背景情况:

自体抗体是自身免疫性肝炎 (AIH) 的关键指标,但大多数缺乏疾病特异性.
溶性肝抗原 (SLA) 和肝脏和胰腺 (LP) 的自身抗体被认为是AIH特异性的,但缺乏标准化测试.
在约30%的AIH患者中发现了SLA和LP自身抗体.

研究的目的:

为了确定SLA/LP自身抗体的特定向抗原.
为了使AIH的标准化诊断试验的发展.
调查SLA/LP抗原在AIH病原发生中的作用.

主要方法:

使用抑制ELISA对2000个血清样本进行了SLA自身抗体的选.
用阳性血清样本对cDNA表达库进行免疫查.
通过表达克隆,吸收实验,删除突变物和外核酶消化,识别和表征目标抗原.

主要成果:

发现SLA和LP的自身抗体是相同的.
一种422氨基酸蛋白质,具有两种拼接变体,被确定为唯一的向抗原.
氨基酸371-409对于SLA/LP抗原的免疫识别至关重要.

结论:

识别的cDNA编码了SLA/LP自身抗体的主要向抗原.
SLA/LP自身抗体是疾病特异性的,识别主导性表位,这表明AIH的抗原驱动免疫反应.
鉴定SLA/LP抗原为可靠的诊断试验和对AIH病原性的进一步研究铺平了道路.

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