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在斑马鱼胃化过程中,Silberblick/Wnt11调解了收的延伸运动

C P Heisenberg¹, M Tada, G J Rauch

¹Department of Anatomy and Developmental Biology, University College, London, UK. c.heisenberg@ucl.ac.uk

|May 16, 2000

概括

斑马鱼的胃化需要Wnt11来协调细胞运动. Wnt11缺乏导致中线缺陷,突出其在缩延伸和脊椎动物发育中的作用.

科学领域:

发育生物学是发展生物学.
分子遗传学分子遗传学
细胞生物学细胞生物学

背景情况:

脊椎动物的胃化通过细胞特异和迁移建立了胚胎层.
调节细胞身份的基因已知,但控制细胞运动的基因理解较少.

研究的目的:

为了识别在脊椎动物胃流动期间协调细胞运动的基因.
为了阐明斑马鱼silberblick (slb) 位点在胃化中的作用.

主要方法:

斑马鱼silberblick (slb) 位点的遗传分析.
功能性研究 Wnt11 和 Dishevelled 在胃流动.
在胃流动过程中对细胞非自主信号的研究.

主要成果:

银眼 (slb) 位置编码为Wnt11,对于收扩展至关重要.
由于异常的轴向延伸,Wnt11功能的丧失导致循环失常和中线缺陷.
Wnt11的要求是细胞非自主,涉及中侧细胞间隔.
一个截断的Dishevelled拯救了slb表型,表明非正规的Wnt信号.

结论:

在脊椎动物胃流动期间,Wnt11对于收的延伸运动至关重要.
侧面组织中的Wnt信号通过细胞间隔驱动胃化运动.
通过Wnt介导的形态发生可能涉及不同的信号传导途径.

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