在Xenopus gastrulation过程中,失调控制了细胞极性
J B Wallingford1, B A Rowning, K M Vogeli
1Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.
Nature
|May 16, 2000
概括
Xenopus Dishevelled (Xdsh) 控制细胞极性,这对胚胎发育至关重要. 这项研究揭示了Xdsh通过维持细胞极性来调节胃流动期间的细胞运动,这对脊椎动物胚胎发生至关重要.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 细胞运动对于胚胎发育至关重要,但它们在脊椎动物中的调节仍然不太清楚.
- 抑制Xenopus Dishevelled (Xdsh) 干扰了胃化细胞的运动,但根本的机制尚不清楚.
- 在Drosophila中已知Dishevelled在细胞极性中的作用,但其在脊椎动物细胞极性中的功能尚未研究.
研究的目的:
- 为了研究Xenopus Dishevelled (Xdsh) 调节胃流动期间细胞运动的机制.
- 要确定Xdsh是否控制脊椎动物胚胎中的细胞极性.
- 阐明两极分化的细胞行为在趋同延伸运动中的作用.
主要方法:
- 时间延迟共聚焦显微镜用于观察Xenopus胚胎中的细胞行为.
- 对影响收延伸的Xdsh突变的分析.
- 研究Xdsh在背面半皮细胞中的定位.
主要成果:
- 缺乏Xdsh功能导致细胞极性缺陷,导致收延伸失败.
- 特定的Xdsh突变与Drosophila Dishevelled突变相关,影响平面细胞极性.
- 在正常的背面半皮细胞中,xdsh定位在细胞膜上,支持其在极性中的作用.
结论:
- 两极化的细胞行为对于脊椎动物胃流动期间的收延伸至关重要.
- 脊椎动物脱骨 (Xdsh) 控制细胞极性.
- 一个类似于Drosophila平面细胞极性通路的信号级联可能是脊椎动物胃流的要求.
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