相关实验视频
Updated: Jul 6, 2026
14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
通过差异化的TAF招募,不同类型的酵母促进剂被揭示出来
X Y Li1, S R Bhaumik, M R Green
1Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Science (New York, N.Y.)
|May 20, 2000
概括
这项研究揭示了两个酵母促进剂类:TAF独立 (TAFind) 和TAF依赖 (TAFdep). TFIID的招聘不同,TBP仅在TAFind发起人处具有约束力,但在TAFdep发起人处共招聘TAF.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 转录条例 转录条例 转录条例
背景情况:
- 转录因子TFIID对于基因表达至关重要.
- TFIID包括TATA盒结合蛋白 (TBP) 和TBP相关因子 (TAF).
- 在不同的发起人类型的TFIID招聘中,TAFs的具体作用尚未完全理解.
研究的目的:
- 研究TFIID成分与酵母中TAF依赖 (TAFdep) 和TAF独立 (TAFind) 促进者的差异关联.
- 阐明基于发起人分类的TFIID招聘机制.
主要方法:
- 对TFIID成分与酵母中的TAFdep和TAFind促进剂的关联分析.
- 评估不同促进者类的TAF和TBP水平.
- 在一般转录因子失活后,激活剂对TAF招募的研究.
主要成果:
- 在TAFdep发起者中,TAFs的存在水平与TBP相当.
- 在TAFind发起人那里,TAFs几乎存在于背景水平.
- 激活器可以招募TAF到TAFdep促进器,即使在TBP失活后.
结论:
- 酵母促进者可以根据TFIID招聘分为TAFdep和TAFind类别.
- 在TAFind的发起人那里,TBP是独立于TAF招募的.
- 在TAFdep促进者中,TAFs与TBP一起被招募,这表明直接的激活器-TAF相互作用.
相关概念视频
01:28Yeast Signaling
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
01:58Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
02:40The Eukaryotic Promoter Region
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
01:58Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
02:40The Eukaryotic Promoter Region
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...