来自预先孕患者的AT(1) 受体激素抗体会导致血管细胞表达组织因子
R Dechend1, V Homuth, G Wallukat
1Franz Volhard Clinic and Max Delbrück Center for Molecular Medicine, Medical Faculty of the Charité, Humboldt University of Berlin, Germany.
针对血管酶-1受体 (AT(1) -AA) 的自身抗体刺激血管细胞表达组织因子 (TF),可能有助于孕前. 这项研究证明了AT(1) -AA对人体细胞的作用,将其与孕前病原发生联系起来.
科学领域:
- 心血管生物学 心血管生物学
- 生殖医学 生殖医学
- 免疫学 免疫学 免疫学
背景情况:
- 孕前与抗抗胰岛素-1受体 (AT(1) -AA) 的自身抗体有关.
- 前性胎盘表达组织因子 (TF),但AT的作用在这种表达是未知的.
- 这项研究调查了AT(1) -AA是否诱导血管光滑肌细胞 (VSMC) 中的TF表达.
研究的目的:
- 为了测试AT(1) -AA导致VSMC表达TF的假设.
- 阐明涉及AT(1) -AA诱导TF表达的信号通路.
- 探索AT(1) -AA对孕前病原的潜在贡献.
主要方法:
- 从孕前患者的IgG和AT(1) -AA的净化.
- 用TF促进剂-露西法酶构造物感染细胞.
- 检测细胞外信号相关激酶 (ERK) 和AP-1激活.
- 通过ELISA和免疫组织化学测量TF表达.
- 使用洛萨坦的抑制研究.
主要成果:
- 孕前的胎盘显示出明显高于对照组的TF染色.
- AT(1) -AA 特别与AT(1) 受体结合.
- ангиотензинII和AT(1)-AA都激活了VSMC和转染细胞中的ERK,AP-1和TF促进体.
- 在VSMC中,TF表达是由Ang II或AT(1)-AA诱导的,这些效应被洛萨坦阻断.
结论:
- AT(1) -AA和Ang II刺激AT(1) 受体,启动信号级联导致TF表达.
- 在人体细胞中,这种AT(1) -AA诱导的TF表达可能在孕前的发展中起作用.
- 这些发现提供了一种潜在的机制,将自身抗体与孕前病原体联系起来.
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