fw2.2:一个定量特征位置的关键,对番茄果实大小的演变
A Frary1, T C Nesbitt, S Grandillo
1Department of Plant Breeding and Department of Plant Biology, 252 Emerson Hall, Cornell University, Ithaca, NY 14853, USA.
Science (New York, N.Y.)
|July 7, 2000
概括
该fw2.2基因,ORFX,通过控制早期花发育期间的骨细胞数量,显著影响番茄果实大小. 化可能涉及ORFX的监管变化,而不是蛋白质改变,以获得更大的果实.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 进化生物学是进化的生物学.
背景情况:
- 植物化往往会导致水果大小的增加.
- 定量特征位点 (QTL) fw2.2是化期间番茄果实大小增加的一个关键因素.
研究的目的:
- 为了确定负责fw2.2 QTL效应的特定基因.
- 了解这种基因影响番茄果实大小的机制.
主要方法:
- fw2.2地区的科斯米德转化为大果番茄品种.
- 在花发育期间对基因表达的分析.
- 对已识别的基因进行序列分析.
- 与人类瘤基因序列的比较.
主要成果:
- 一个单一的基因ORFX被确定为 fw2.2 QTL 效应的原因.
- ORFX在花发育的早期表达,并控制骨细胞数量.
- ORFX序列与人类癌基因c-H-ras p21相似.
- 与 fw2.2 区域的转变使得果实大小如预测的那样减少.
结论:
- fw2.2 QTL效应主要是由于ORFX基因.
- 果实大小的改变很可能是由基因调节的变化引起的,而不是蛋白质序列的变化.
- ORFX在水果发育和化中起着至关重要的作用.
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