6SRNA调节大肠杆菌的RNA聚合酶活动
1Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA. kwass@box-k.nih.gov
Cell
|July 13, 2000
概括
发现了大肠杆菌6SRNA的功能:它与RNA聚合酶结合,特别是sigma70和β/ββ.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 基因法规 基因法规
背景情况:
- 30多年前发现的大肠杆菌6SRNA的功能在很大程度上仍然未知.
- 了解6SRNA的作用对于破译细菌基因调节至关重要.
研究的目的:
- 为了阐明大肠杆菌6SRNA的功能.
- 为了研究6SRNA和RNA聚合酶之间的相互作用.
主要方法:
- 紫外线交叉连接实验以确定6SRNA和RNA聚合酶子单元之间的直接接触.
- 在不同生长阶段对6SRNA积累的分析.
- 测试以确定6SRNA对sigma70-holenzyme活性和促进体抑制的影响.
主要成果:
- 6S RNA 特定且有效地与RNA聚合酶结合.
- 证实了6SRNA与RNA聚合酶的sigma70和β/β'子单元之间的直接接触.
- 在静止阶段,6SRNA水平增加,改变RNA聚合酶活性.
- 6SRNA对于sigma70与核心RNA聚合酶的稳定结合至关重要.
- 在静止阶段,6S RNA抑制了来自sigma70依赖的促进体的转录.
结论:
- 在特定的生长阶段,6SRNA在调节RNA聚合酶活性方面发挥着关键作用.
- 6S RNA与RNA聚合酶的相互作用通过影响sigma70-holoenzyme功能来调节基因表达.
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