Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Lethal Alleles02:41

Lethal Alleles

Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Single-cell tumor-immune microenvironment of BRCA1/2 mutated high-grade serous ovarian cancer.

Nature communications·2022
Same author

Detecting episomal or integrated human papillomavirus 16 DNA using an exonuclease V-qPCR-based assay.

Virology·2019
Same author

Depletion of florfenicol in lactating dairy cows after intramammary and subcutaneous administration.

Journal of veterinary pharmacology and therapeutics·2016
Same author

Generation of high-titer, helper-free retroviruses by transient transfection.

Methods in molecular medicine·2014
Same author

Mass spectrometry based method to increase throughput for kinome analyses using ATP probes.

Analytical chemistry·2013
Same author

The cancer biology of whole-chromosome instability.

Oncogene·2013

相关实验视频

Updated: Jul 11, 2026

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
13:59

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

在缺乏线粒检查点蛋白 Mad2 的小鼠中,染色体误分和亡.

M Dobles1, V Liberal, M L Scott

  • 1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.

Cell
|July 13, 2000
PubMed
概括

螺旋组装检查点蛋白 Mad2 对于小鼠细胞中精确的染色体分离至关重要. 它的缺失导致细胞分裂和胚胎死亡的错误,突显了它在防止线粒学灾难方面的重要性.

科学领域:

  • 细胞生物学 细胞生物学
  • 遗传学 是一个遗传学.
  • 发展生物学 发展生物学

背景情况:

  • 螺旋组合检查点 (SAC) 通过将无相启动与适当的染色体-微管附着连接,确保准确的染色体分离.
  • 麦德2 (MAD2) 是SAC中的关键蛋白质,但其在哺乳动物发育中的确切作用尚未完全理解.

研究的目的:

  • 研究Mad2在正常细胞分裂过程中的功能以及对小鼠的线粒应激反应.
  • 确定Mad2缺乏对胚胎发育和染色体分离的影响.

主要方法:

  • 一代的Mad2淘汰赛小鼠.
  • 在Mad2-缺陷胚胎中分析胚胎细胞分裂,染色体分离和亡.
  • 线粒细胞和线粒后细胞种群的比较.

主要成果:

  • 缺少Mad2的胚胎细胞 (E5.5) 正常分裂,但在线索中断时无法停止.
  • 到E6.5,缺乏Mad2的表皮质细胞由于检查点控制失败而表现出广泛的染色体误分和亡.
  • 缺少Mad2的胚胎中的转移后的巨细胞存活下来,这表明细胞类型对SAC的特定要求.

结论:

  • 由Mad2介导的螺旋组装检查点,对于在分裂的哺乳动物细胞中准确分离染色体至关重要.

更多相关视频

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

相关实验视频

Last Updated: Jul 11, 2026

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
13:59

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

  • 由于Mad2缺乏,即使在没有外部螺旋损伤的情况下,也会导致线粒错误,从而损害胚胎的生存能力.
  • 在小鼠早期发育过程中,SAC在维持基因组稳定性方面发挥着至关重要的作用.