在斑马鱼胚胎中遗传的功能性昼夜时钟
F Delaunay1, C Thisse, O Marchand
1Ecole Normale Supérieure, CNRS UMR 5665, 46 allée d'Italie, 69364 Lyon Cedex, France.
Science (New York, N.Y.)
|July 15, 2000
概括
斑马鱼的昼夜时钟在早期胚胎发生过程中起作用,Period3 (Per3) 基因表现出节奏表达. 母亲的Per3遗传可能解释了时钟是如何通过几代人传承的.
科学领域:
- 发展生物学 发展生物学
- 时间生物学 时间生物学
- 遗传学 遗传学 是一个
背景情况:
- 昼夜时钟是内生计时系统,对于调节大多数生物体的生理过程至关重要.
- 周期3 (Per3) 基因是围绕昼夜节律的分子机械的关键组成部分.
- 了解胚胎发育期间昼夜节律的发生和继承是很重要的.
研究的目的:
- 在早期斑马鱼胚胎发生过程中调查昼夜时钟的功能状态.
- 确定光线和发育温度在重置胚胎昼夜时钟中的作用.
- 探索母亲因素对昼夜节律遗传的贡献.
主要方法:
- 在不同发育阶段的斑马鱼胚胎中对Per3基因表达模式的定量分析.
- 将胚胎暴露在不同的光暗周期和温度条件下,以评估时钟重置.
- 在卵细胞和早期胚胎中分析Per3信使RNA的积累.
主要成果:
- 在斑马鱼胚胎的中枢神经系统和视网膜中,Per3表达表现出强大的昼夜节律.
- 胚胎的Per3节奏是自由运行的,可以被光所吸引,但对低温引起的发育延迟不敏感.
- Per3信使RNA在卵细胞中节律积累,并在早期胚胎中持续存在,表明母亲的贡献.
结论:
- 昼夜时钟在斑马鱼早期胚胎发生过程中是功能和操作的.
- 光作为胚胎时钟的主要Zeitgeber,而发育温度不会重置它的阶段.
- 时钟基因产品的母体遗传,如Per3,可能提供了通过 ovogenesis 阶段遗传的机制.
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