Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Tissue Transplantation

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Innovative 3D-printed internal structure in silicone partial-hand prostheses: Enhancing function and mechanical performance.

Canadian prosthetics & orthotics journal·2026

Same author

Reliability and Validity Testing of the Persian Version of the Derriford Appearance Scale 24 in a Sample of Individuals with Craniofacial Irregularity and Amputation.

Canadian prosthetics & orthotics journal·2024

Same author

Extracorporeal Membrane Oxygenation as a Bridge to Lung Transplantation in Patients Over Age 70 Years: A Case Report.

Transplantation proceedings·2017

Same author

Current outcome of heart transplantation: a 10-year single centre perspective and review.

QJM : monthly journal of the Association of Physicians·2010

Same author

Differential expression of enteric neuroimmune-network in invasive and acute watery diarrhoea.

Neurogastroenterology and motility·2009

Same author

Left ventricular assist devices.

Heart (British Cardiac Society)·2009

Search research articles

相关实验视频

Updated: Jun 9, 2026

Murine Heterotopic Heart Transplant Technique

Murine Heterotopic Heart Transplant Technique

Published on: July 9, 2014

瘤亡因子-α在供体心脏中表达,并预测人心移植后的右心室衰竭.

E J Birks¹, V J Owen, P B Burton

¹National Heart and Lung Institute at the Imperial College School of Medicine, Royal Brompton and Harefield Hospital, Harefield, Middlesex, UK.

|July 19, 2000

概括

瘤亡因子-α (TNF-α) 在供体心肌中预测心脏移植后的早期右心室衰竭. 在捐赠心脏中检测TNF-alpha可以识别患有这种并发症高风险的患者.

更多相关视频

Study of Experimental Organ Donation Models for Lung Transplantation

Study of Experimental Organ Donation Models for Lung Transplantation

Published on: March 15, 2024

A Neonatal Heterotopic Rat Heart Transplantation Model for the Study of Endothelial-to-Mesenchymal Transition

A Neonatal Heterotopic Rat Heart Transplantation Model for the Study of Endothelial-to-Mesenchymal Transition

Published on: July 21, 2023

相关实验视频

Last Updated: Jun 9, 2026

Murine Heterotopic Heart Transplant Technique

Murine Heterotopic Heart Transplant Technique

Published on: July 9, 2014

Study of Experimental Organ Donation Models for Lung Transplantation

Study of Experimental Organ Donation Models for Lung Transplantation

Published on: March 15, 2024

A Neonatal Heterotopic Rat Heart Transplantation Model for the Study of Endothelial-to-Mesenchymal Transition

A Neonatal Heterotopic Rat Heart Transplantation Model for the Study of Endothelial-to-Mesenchymal Transition

Published on: July 21, 2023

科学领域:

心脏病学心脏病学
免疫学免疫学免疫学
移植医学移植医学

背景情况:

右心室 (RV) 衰竭是心脏移植后的常见并发症,其机制尚不清楚.
炎症性细胞因子与心力衰竭的发病有关.
这项研究调查了瘤亡因子-α (TNF-α) 和其他细胞因子在供体心肌中的作用.

研究的目的:

检查捐赠心脏中TNF-alpha和其他细胞因子的表达.
确定细胞因子表达和接受者早期RV衰竭的发展之间的关系.
评估TNF-alpha作为RV衰竭的预测生物标志物.

主要方法:

在26名捐赠者中使用过食道心声学评估腹腔功能.
通过RT-PCR获得的供体RV活检以分析细胞因子mRNA表达 (TNF-alpha,IL-2,IFN-gamma,iNOS).
使用西式抹杀量化TNF-α蛋白水平,并使用免疫细胞化学定位其表达.
随访接受者进行了移植后RV衰竭的发展.

主要成果:

26名接受者中有8人 (30.8%) 发展为RV衰竭.
在87.5%的接受者患有VR衰竭的捐赠者中检测到TNF-αmRNA,而在没有RV衰竭的捐赠者中,这一比例为22.2% (P<0.005).
TNF-alpha mRNA显示了VR失败的高预测值 (灵敏度为87.5%,特异性为83.3%).
在随后出现RV衰竭的捐赠者的心肌中发现了更高的TNF-α蛋白水平 (P<0.05).

结论:

在心脏肌细胞中,捐赠心脏TNF-alpha表达是心脏移植后早期RV衰竭的重要预测因素.
这一发现表明TNF-alpha可能在RV衰竭的发展中起着至关重要的作用.
在捐赠心脏中早期检测TNF-alpha可能有助于心脏移植接受者的风险分层.